2型糖尿病患者外周血单核细胞血红素氧合酶-1表达水平与氧化应激的相关性  

Relationship between the expression of heme oxygenase-1 in the peripheral blood mononuclear cell and oxidative stress in newly diagnosed type 2 diabetes

作　　者：邹艺[1] 薛耀明[1] 易正山[2] 沙建平[1] 卓凤婷[1] 曾展军[1] 

机构地区：[1]南方医科大学南方医院内分泌代谢科,广州市510515 [2]南方医科大学南方医院血液科,广州市510515

出　　处：《实用医学杂志》2008年第17期2972-2974,共3页The Journal of Practical Medicine

摘　　要：目的:探讨初诊的2型糖尿病患者机体的氧化应激状态,以及患者外周血单核细胞(peripheral blood mononuclear cell,PBMC)血红素氧合酶-1(heme oxygenase-1,HO-1)mRNA的表达与机体氧化应激状态的关系。方法:应用流式细胞仪检测22例初诊2型糖尿病患者(糖尿病组)细胞内活性氧(reactive oxygen species,ROS)产量。硫代巴比妥酸比色法检测血清中丙二醛(malondialdehyde,MDA)含量,逆转录聚合酶链反应技术分析PBMC中HO-1mRNA表达水平,并与22名健康对照者(对照组)比较。结果:糖尿病组PBMC中ROS产量测定的平均荧光强度(mean fluorescence intensity,MFI)为50.36±21.69,与对照组28.80±10.14比较,差异有显著性(P<0.01)。糖尿病组血清中MDA含量测定为(14.47±5.21)nmol/mL,与对照组的(5.57±1.21)nmol/mL比较,差异亦有显著性(P<0.01)。糖尿病组PBMC中HO-1/β-actin mRNA基因表达率的平均值为0.98±0.24,与对照组0.48±0.18比较,差异有显著性(P<0.01)。HO-1 mRNA的表达水平与ROS的产量、MDA含量均呈显著正相关(r=0.491、0.745,均P<0.01)。结论:初诊的2型糖尿病患者处于氧化应激状态,其中HO-1表达水平的增高可能参与2型糖尿病的抗氧化应激损伤。Objective To investigate the change of oxidative stress and expression of heme oxygenase-1 （HO- 1） in the peripheral blood mononuclear cell （PBMC） of newly diagnosed type 2 diabetes. Methods Twenty-two newly diagnosed type 2 diabetes and twenty-two healthy subjects were selected. The intracellular reactive oxygen species（ROS） production were detected by flow cytometry, serum contents of malondialdehyde （MDA） were measured with TBA method. HO-1 mRNA PBMC were determined with reverse transcription-polymerase chain reaction in newly diagnosed type 2 diabetes and healthy subjects. Results The intracellular ROS production were significantly higher in newly diagnosed type 2 diabetes （50.36 ± 21.69） compared with that of healthy subjects （28.80 ± 10.14 , P 〈 0.01）. MDA concentration in newly diagnosed type 2 diabetes[ （14.47 ± 5.21 ）nmol/mL] was significantly higher than that in healthy subjects[（5.57 ± 1.21 ）nmol/mL, P 〈 0.01）. The gene expression ration of PBMC HO-1/β-actin mRNA were significantly higher in newly diagnosed type 2 diabetes than in healthy controls （0.98 ± 0.24 vs 0.48 ± 0.18, P 〈 0.01）. The correlation analysis showed that PBMC HO-1 mRNA levels had significantly positive relation with intracellular ROS production, contents of MDA（r=0.491,0.745, P 〈 0.01 respectively）. Conclusions Oxidative stress is a major contributor to the pathogenesis of type 2 diabetes. The augment of oxidative stress associated with expression of HO-1 may be involved in the pathogenesis of type 2 diabetes.

关 键 词：糖尿病 2型 血红素氧化酶(脱环) 单核细胞 活性氧 丙二醛 

分 类 号：R587.1[医药卫生—内分泌]