P2Y_(12)受体基因多态性在中国人群中的分布和对氯吡格雷疗效的影响  

P2Y_(12) gene polymorphisms in Chinese healthy population and patients with coronary artery disease and the association with effect of clopidogrel

作　　者：陈碧莲[1] 李清[2] 解勤之[3] 谢伟[4] 马琦琳[4] 

机构地区：[1]中南大学湘雅医院干部医疗科,长沙410008 [2]中南大学临床药理研究所,长沙410008 [3]中南大学湘雅医院血液科,长沙410008 [4]中南大学湘雅医院心血管内科,长沙410008

出　　处：《中国新药杂志》2008年第16期1435-1439,共5页Chinese Journal of New Drugs

摘　　要：目的:探讨P2Y12受体C34T和G52T基因多态性在中国汉族健康人群与冠心病患者中的分布特点,并进一步分析对氯吡格雷药物疗效的影响。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测190例冠心病患者和240例健康对照组的P2Y12受体C34T和G52T基因多态性,选取健康受试者18例(6例P2Y12CC34/GG52,6例P2Y12TT34/GG52和6例P2Y12CC34/TT52),测定服用氯吡格雷前后血小板聚集率。结果:P2Y12受体C34T和G52T基因突变频率在中国健康人群和冠心病患者中分别是24.2%,12.1%和21.1%,10.5%,但2个位点突变频率在2组人群中无明显差异(P>0.05)。在3种P2Y12单倍型之间,服用氯吡格雷前后血小板聚集率无明显差异(P>0.05)。结论:P2Y12受体C34T和G52T基因多态性在中国汉族健康人群和冠心病患者中分布无明显差异,并且对氯吡格雷抗血小板聚集率的疗效无明显影响。Objective：To investigate the P2Y12 receptor gene polymorphisms in haM Chinese healthy population and patients with coronary artery disease, and the association of polymorphisms with the effect of clopidogrel. Methods： In 190 patients with coronary artery disease and 240 healthy controls, P2Y12 C34T and G52T gene polymorphisms were analyzed by polytherase chain reaction and restriction fragment length polymorphism （PCR-RFLP） assay. Platelet aggregation was measured during clopidogrel administration in 18 healthy subjects （including 6 P2Y12 CC34/GG52, 6 P2Y12 TT34/GG52 and 6 P2Y12 CC34/TT52）. Results： In healthy population and patients with coronary artery disease, the frequency of P2Y12 C34T gene mutations was 24.2% and 21.1% , and that of G52T gene mutations was 12.1% and 10.5%. No significant difference of these gene mutations was observed between the two populations （P 〉 0.05）. Among three different P2Y12 genotypes, there was no significant difference in platelet aggregation during clopidogrel administration （P 〉0.05）. Conclusion： The frequencies of P2Y12 C34T and G52T gene polymorphisms are similar in han Chinese healthy population and patients with coronary artery disease. These polymorphisms do not affect the anti-platelet aggregation activity of clopidogrel.

关 键 词：P2Y12 基因多态性 冠心病 氯吡格雷 

分 类 号：R969.1[医药卫生—药理学] R973.2[医药卫生—药学]