表皮生长因子对骨髓间充质干细胞体外趋化的影响  

The Effect of Epidermal Growth Factor on Chemotaxis of Bone Marrow Mesenchymal Stem Cells in Vitro

作　　者：高志强[1] 程鹏[2] 刘云会[1] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110001 [2]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《解剖科学进展》2008年第3期237-239,243,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(No.30570650;30700861;30670723);辽宁省自然科学基金(No.20052102)

摘　　要：目的观察表皮生长因子(EGF)对骨髓间充质干细胞(BMSCs)的体外趋化的影响,探讨EGF对BMSCs迁移影响的可能机制。方法密度梯度离心法分离BMSCs,体外培养、传代纯化。利用Transwell小室建立体外趋化迁移模型。分别观察0,25,50,75,150ng/ml浓度的EGF对BMSCs的体外趋化作用,以及加入EGF中和抗体后对BMSCs趋化作用的影响;应用反转录聚合酶链反应(RT-PCR)方法检测大鼠BMSCs有无表皮生长因子受体(EGFR)的表达。结果细胞迁移实验结果:EGF以浓度梯度(0,25,50,75,150ng/ml)依赖方式促进大鼠BMSCs的定向迁移,作用显著(P<0.05)。抗体中和后的迁移实验:75ng/ml的EGF对BMSCs的趋化作用可被EGF中和抗体明显减弱(P<0.01)。RT-PCR证实BMSCs表达EGFR。结论在体外,EGF以浓度依赖方式引起BMSCs的定向迁移,BMSCs表达EGF的受体EGFR。Objective To investigate the effect of epidermal growth factor （ EGF ） on the migration of mesenchymal stem cells （ BMSCs ） and to explore the possible mechanism of this effect. Methods BMSCs were isolated, cultured, passaged and purified in vitro by density gradient centrifugation method. Migration and chemotaxis mice models were established using transwell inserts technique.The biological effect of EGF （ 0,25,50,75 and 150ng/ml ） on BMSCs was observed.The chemotaxis in vitro of MSCs by EGF of the above concentrations was observed by cell migration test.After its neutral antibody was added,the effect of EGF on BMSCs chemotaxis was observed. The expression of EGF receptor was examined by RT-PCR method. Results In the cell migration test, EGF improved the directional migration of rat BMSCs significantly in the manner of concentration dependence with the concentration gradient of 0, 25, 50, 75 and 150ng/ml （ P〈0.05 ） . The migration of BMSCs induced by EGF with the concentration of 75ng/ml was depressed significantly by EGF neutral antibody （ P〈0.01 ） . EGFR mRNA was expressed in BMSCs by RT-PCR method. Conclusion EGF enhances directional migration of rat BMSCs in vitro in a concentration-dependence manner, BMSCs expresses EGFR.

关 键 词：骨髓间充质干细胞 趋化作用 表皮生长因子 表皮生长因子受体 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学] Q257[医药卫生—基础医学]