大鼠骨髓间充质干细胞向神经细胞和星形胶质细胞分化的研究  

Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells and Astrocytes in Rats

作　　者：张海燕[1] 王健[1,2] 卢玉仙[1] 胡勇[3] 夏春林[1] 

机构地区：[1]苏州大学医学部人体解剖学暨神经生物学教研室 [2]江苏省建湖县人民医院骨科,江苏建湖224700 [3]江苏省建湖县人民医院骨科

出　　处：《解剖科学进展》2008年第3期295-297,301,共4页Progress of Anatomical Sciences

基　　金：江苏高校自然科学研究计划(05KJB310121)

摘　　要：目的探讨体外诱导骨髓间充质干细胞(BMSCs)分化成神经细胞或星形胶质细胞。方法使用四种不同的诱导方式来处理BMSCs:-巯基乙醇(BME)、维甲酸(RA)、无血清培养基以及和新生鼠的脑星形胶质细胞共培养。结果四种方法处理后,BMSCs都能表达神经细胞标示物MAP2或星形胶质细胞标志物GFAP,并伴随着明显的细胞形态的改变。用RA诱导以及在无血清的培养基中培养,BMSCs主要分化成神经元样细胞,表达神经元的标志物MAP2,并且BME能促进这个过程。但在和星形胶质细胞共培养后,BMSCs主要分化成GFAP+细胞。结论BMSCs依赖于不同的诱导条件,具有分化成神经细胞或星形胶质细胞的可塑性。Objective To investigate the differentiation of bone marrow mesenchymal stem cells （BMSCs） into neural cells or astrocytes in vitro. Methods We cultured the BMSCs with four different treatments： mercaptoethanol （BME）, ratinoic acid （RA）, serum-free medium and co-cuhivation with fetal mouse brain astrocytes. Results In all of the treatments, BMSCs could express neural markers of microtubule-associated protein 2 （MAP2） or glial fibrous acidic protein （GFAP）, associating with remarkable cytomorphological modifications. But these treatments led to neural phenotype in a nonidentical manner. BMSCs mainly differentiated into neuron-like cells with MAP2 positive by RA or serum-free medium, and BME can promote this process. Differently, after co-culturing with astrocytes, BMSCs leaned to differentiate into GFAP cells. Conclusion BMSCs can be differentiated into neural cell or astrocyte in vitro, depending on the protocols of inducement.

关 键 词：骨髓间充质干细胞 神经细胞 星形胶质细胞 大鼠 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]