少突胶质细胞与2型星形胶质细胞中S-100β表达差异性的研究  

The Expression of S-100β in Oligodendrocytes and Type-2 Astrocytes

作　　者：卢玉仙[1] 张海燕[1] 刘珺[1] 孙茂民[1] 夏春林[1] 

机构地区：[1]苏州大学医学部,人体解剖学暨细胞神经生物学教研室,江苏苏州215123

出　　处：《解剖科学进展》2008年第3期309-311,共3页Progress of Anatomical Sciences

基　　金：江苏省高校自然科学研究计划(05KJB310121)

摘　　要：目的研究少突胶质细胞(oligodendrocytes,OL)与2型星形胶质细胞(type-2astrocyte,T2A)中S-100的表达。方法根据各种胶质细胞的生长时间的差异及细胞的黏附性不同,通过机械振荡法和差速贴壁法获得纯化的O-2A祖细胞;免疫细胞化学染色法观察S-100β在O-2A谱系细胞中的表达情况。结果O-2A祖细胞在含有PDGF、bFGF的培养液中培养3d,分化成T2A,培养5d分化成OL;OL中S-100β免疫反应呈现阳性,T2A中不表达S-100β。结论O-2A谱系细胞中的S-100β的表达与O-2A祖细胞向OL分化有关。Objective To investigate the expression of S-100βin the oligodendrocytes（OL） and type-2 astrocyte（T2A）. Methods Based on the differential properties of cellular adhesions and developmental time-course, O-2A progenitor cells were obtained by mechanical shaking and differential adhesion method. S-100β expression was detected by immunocytochemistry staining. Results O-2A progenitor cells were cultured and differentiated into oligodendrocytes （5d culture） and type-2 astroeytes （3d culture） in the presence of PDGF and bFGF. However, S-100 β expression was found in oligodendrocytes, but not seen in type-2 astrocytes. Conclusion The expression of S-100 β in O-2A lineage cells may be correlatd with the differentiation of the O-2A progenitor cells into the OL.

关 键 词：O-2A祖细胞 少突胶质细胞 2型星形胶质细胞 S-100Β 

分 类 号：Q257[生物学—细胞生物学] R329.23[医药卫生—人体解剖和组织胚胎学]