纳米组织工程骨与兔骨髓基质干细胞体外生物相容性的实验研究  

Biocompatibility of Nano-Hap/Collagen and Rabbit Bone Mesenchymal Stem Cells

作　　者：王明海[1] 董有海[1] 冯庆玲[2] 刘新晖[3] 洪洋[1] 顾军[1] 吴俊国[1] 王晓平[1] 童文卿[1] 

机构地区：[1]复旦大学附属上海市第五人民医院骨科,200240 [2]清华大学材料科学与工程系 [3]河北医科大学第一医院骨科

出　　处：《中国骨与关节损伤杂志》2008年第9期745-747,共3页Chinese Journal of Bone and Joint Injury

基　　金：上海市科委科技攻关计划资助项目(064119649);复旦大学青年科学基金资助项目(JKF161001)

摘　　要：目的探讨纳米组织工程骨(NHAC)与兔骨髓基质干细胞(RMSCs)体外生物相容性,为应用组织工程方法修复骨缺损提供依据。方法将RMSCs与NHAC体外复合培养,进行形态学和功能测定。结果骨髓基质干细胞能在纳米组织工程骨上良好地粘附、增殖、生长。细胞的活性和碱性磷酸酶活性未受到纳米组织工程骨的影响。结论NHAC具有良好的生物相容性,可作为骨组织工程理想的生物材料。Objective To explore the biocompatibility of the nano- hydroxyapatite/collagen composite and rabbit bone mesenchymal stem cell in order to offer the ideal bone material for treating bone defect. Methods The morphology and function of the RMSCs were observed by coculturing NHAC with RMSCs in vitro. Results The RMSCs could well adhere to, proliferate and grow normally and perfectly on the surface and holes of NHAC. The cellular activity and alkaline phosphatase activity were not influenced by NHAC. Conclusion NHAC has good biocompatibility and can be used as ideal biomaterial in bone tissue engineering.

关 键 词：纳米组织工程骨 骨髓基质干细胞 生物相容性 

分 类 号：R318[医药卫生—生物医学工程] R-332[医药卫生—基础医学]