刚竹属3种重要散生竹光系统Ⅰ基因(Lhca1)的克隆、序列分析和蛋白结构预测  

Cloning and sequence analysis of the light harvesting complex Ⅰ gene(Lhca1) from three kinds of Phyllostachys and structure prediction of the protein

作　　者：唐文莉[1] 彭镇华[1] 高健[1] 

机构地区：[1]国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室

出　　处：《北京林业大学学报》2008年第4期109-115,共7页Journal of Beijing Forestry University

基　　金：“948”国家林业局引进项目(2005-4-38、2004-4-60);“十一五”国家科技支撑计划课题(2006BAD19B0203);国家人事部留学回国人员科技择优资助项目

摘　　要：光系统Ⅰ(PSⅠ)在植物光合系统中具有重要功能,Lhca1是编码PSⅠ复合物中最主要的捕光色素蛋白LHCⅠ的基因。该研究采用RT-PCR法,从毛竹中克隆了捕光叶绿素a/b结合蛋白基因LhcaPe01(GenBank EU035496)的全长,从红壳竹和角竹中克隆了长度分别为616、613 bp的捕光叶绿素a/b结合蛋白基因片段LhcaH01(GenBank EU513200)、LhcaJ01(GenBank EU513201)。运用生物信息学方法对其核苷酸序列、编码的氨基酸序列以及蛋白结构进行预测。结果表明,LhcaPe01基因序列从第39 bp开始到第779 bp含有1个开放阅读框和一个中止密码子,该基因全长1051 bp,在5′端有38 bp的非编码区,在3′端含有242 bp的非编码区和Poly(A)30 bp。对这3个基因的保守片段的序列分析表明,刚竹属3种竹种毛竹、红壳竹和角竹保守区内核酸序列同源性非常高,在禾本科内不同属之间毛竹与大麦序列同源性最高,玉米次之。编码的氨基酸序列同源性与核酸序列同源性一致,最高是毛竹与大麦,水稻次之。经推测LhcaPe01编码的蛋白质等电点和分子量分别为5.4000和22107.34 D。蛋白质结构预测表明,毛竹、大麦、水稻、玉米的LHCⅠ蛋白结构非常相似。Light harvesting complex I （LHC Ⅰ ）, is a chlorophyll a/b-binding protein, which plays a key role in plant photosynthesis. A full-length cDNA encoding Lhcal gene was cloned from the first strand of Phyllostachys edulis cDNA through RT-PCR method, named as LhcaPeO1 （GenBank EU035496）. 616 bp cDNA fragment is cloned from the first strand of P. iridescens cDNA, named as LhcaHO1 （GenBank EU513200） and 613 bp cDNA fragment is cloned from the first strand of P. fimbriligula cDNA, named as LhcaJO1 （GenBank EU513201）. The length of LhcaPeO1 is 1 051 bp,which contains an open reading frame encoding 249 amino acids from 39 to 779 positions and has 38 bp and 242 bp untranslated regions of 5＇ end and3＇ end, respectively. There is a 30 bp Poly（A） in 3′ end. The pl and Mw of protein encoded by LhcaPeO1 are predicted to be 5.400 0 and 22 107.34 D, respectively. The similarity of LhcaPe01 DNA sequence and encoding amino acid sequence blast with barley is the highest in Gramineae family. In Phyllostachys genus, the Lhcal similarity of P. iridescens, P. fimbriligula and P. edulis is more higher than that in Gramineae family. The prediction of the protein structure indicates that Moso bamboo, barley, rice and maize have high similarity.

关 键 词：毛竹 红壳竹 角竹 Lhca1 克隆 序列分析 结构预测 

分 类 号：S718.3[农业科学—林学] S759