双歧杆菌的完整肽聚糖对小鼠腹腔巨噬细胞膜脂流动性的影响  

Influence of whole peptidoglycan of Bifidobacteria on the membrane lipidic fluidity of murine peritoneal macrophages

作　　者：魏晓霞 李迎雪 王立生 荀安营 朱惠明 马晓东[2] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院消化科,广东深圳518020 [2]南方医科大学中心实验室,广东广州510515

出　　处：《中国微生态学杂志》2008年第4期335-336,共2页Chinese Journal of Microecology

基　　金：广东省自然基金资助课题;编号:04006961;05009077

摘　　要：目的探讨分叉双歧杆菌的完整肽聚糖(WPG)对巨噬细胞膜脂流动性的影响。方法首先分离培养昆明小鼠腹腔巨噬细胞,然后以WPG刺激巨噬细胞,再用细胞膜磷脂荧光探针标记细胞,最后采用激光共聚焦显微镜结合激光漂白后荧光恢复技术检测巨噬细胞的膜脂流动性。结果WPG刺激组反映小鼠腹腔巨噬细胞膜脂流动性的平均荧光恢复率明显高于对照组(P<0.01)。结论分叉双歧杆菌的完整肽聚糖可提高巨噬细胞膜脂流动性。Objective To explore the influence of whole peptidoglycans （WPG） of Bifidobacteria bifidum on the membrane lipidic fluidity of murine peritoneal macrophages. Methods Mouse peritoneal macrophages were separated and cultivated. The macrophages were stimulated by WPG, and labelled by the fluorescence probe of the membrane lipid. The membrane lipidic fluidity of the macrophages was detected by using laser scanning confocal microscopy and fluorescence re- covery after photobleaching. Result The average fluorescence recovery ratio of the macrophages in WPG stimulating group was significantly higher than that in control group. Conclusion WPG of Bifidobacteria bifidum could increase the mem- brane lipidic fluidity of murine peritoneal macrophages.

关 键 词：双歧杆菌 完整肽聚糖 巨噬细胞 膜脂流动性 

分 类 号：R378.992[医药卫生—病原生物学]