右旋糖苷衍生物纳米粒对人乳腺癌细胞的转染作用研究  

Study on the transfection of dextran derivative nanoparticles to the mda-mb-435 human breast cancer cells

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作  者:李明春[1] 梁东升[1] 李友伟[1] 刘莉[1] 

机构地区:[1]解放军第四零一医院药剂科,山东青岛266071

出  处:《中国药理学通报》2008年第8期1019-1022,共4页Chinese Pharmacological Bulletin

基  金:全军"十一五"医药卫生科研基金资助项目(No06ma094)

摘  要:目的以右旋糖苷为起始反应物制备对人乳腺癌细胞具有高转染活性的右旋糖苷衍生物纳米粒非病毒基因载体。方法先将右旋糖苷40与高碘酸钠以不同的配比进行反应,生成醛基含量不同的氧化右旋糖苷;氧化右旋糖苷与精胺以不同的配比反应,共生成9种右旋糖苷衍生物纳米粒;以绿色荧光蛋白基因为报告基因、mda-mb-435人乳腺癌细胞为转染细胞,考察9种合成物的基因转染活性,从中选出转染活性最高的生成物。结果高碘酸钠与右旋糖苷40按摩尔比为0.8∶1反应生成氧化右旋糖苷;该氧化右旋糖苷再与精胺按1∶1的摩尔比继续反应,生成的终产物的转染效率最高。结论制备出了对mda-mb-435人乳腺癌细胞具有高转染活性的右旋糖苷衍生物纳米粒非病毒基因载体。Aim To prepare the dextran derivative nanoparticles with higher transfecting efficiency to mdamb-435 human breast cancer cells. Methods The dextran 40 and the sodium periodate were reacted with different mole ratio to form the intermediate:oxidized dextrans having different aldehyde content. With different reactants matching, the spermine were grafted onto the oxidized dextrans mentioned above to form the dextran-spermine imine conjugates which were reduced by the excess reducing agent NaBH4 to produce the end products 9 species of dextran derivative nanoparticles (extran-spermine amine conjugates). The dextran de- rivative nanipartical of the highest gene transfecting ef- ficiency was selected from the 9 species of dextran derivative nanoparticles by the experiment of gene trans- fection,using the pEGFP-C1 plasmid as the reporter, the mda-mb-435 human breast cancer cells as the transfected cells. Results The dextran derivative nanoparticles with the highest transfecting efficiency was the one that had the highest content of spermine grafted onto the oxidized dextran40 obtained by the reaction between sodium periodate and dextran40 with the mole ratio of 0. 8. Conclusion The dextran derivative nano- particles of the highest transfecting efficiency to the mda-mb-435 human breast cancer had been prepared.

关 键 词:右旋糖苷 精胺 纳米粒 非病毒基因载体 基因转染 

分 类 号:R318.08[医药卫生—生物医学工程] R329.2[医药卫生—基础医学]

 

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