细胞因子诱导杀伤细胞在动物体内的分布  

The distribution of CIK cell after intravenous injection in animals

作　　者：陈绍亮[1] 许兰文[2] 陈雪芬[1] 顾宇参[1] 陈曙光[1] 李蓓蕾[1] 刘文官[1] 石洪成[1] 乔伟伟[2] 吴直江[3] 高奇蓉[3] 

机构地区：[1]复旦大学核医学研究所、复旦大学附属中山医院核医学科,上海200032 [2]复旦大学实验动物科学部 [3]中国科学院上海生命科学研究院生物化学与细胞生物学研究所

出　　处：《中华核医学杂志》2008年第4期233-235,共3页Chinese Journal of Nuclear Medicine

摘　　要：目的观察细胞因子诱导杀伤（CIK）细胞在动物体内分布的特点。方法 ^99Tc^m-标记CIK细胞采用亚锡还原法。昆明种小白鼠40只．按随机数字表法分为8组，每组5只，进行小鼠体内分布实验，计算每克组织百分注射剂量率（％ID／g）。新西兰家兔3只行动态显像，连续观察^99Tc^m-CIK细胞体内分布图像特点。结果分离纯化后^99Tc^m-CIK细胞的放化纯〉95％，室温下6h内保持稳定。，小白鼠体内^99Tc^m-CIK细胞血液清除较快，观察到血液中1和24h的再分布现象。^99Tc^m-CIK细胞主要分布于肺、肝、脾和肾，心脏、脑、骨骼和肌肉等其他脏器组织均分布较少。家兔动态显像示”^99Tc^m-CIK细胞早期分布以肺为主．随时间延长肺内放射性逐渐减少．肝放射性逐渐增多，并超过肺部放射性，2d后仍可见肝放射性浓聚。心脏、脑、骨骼和肌肉等其他脏器组织均未见明显放射性分布。结论^99Tc^m-CIK细胞在血循环中滞留时问较短，注射后1h内主要聚集于肺，随后肺的摄取逐渐减少，其主要浓聚于肝和脾，尤以肝为甚。Objective The aim of this research was to investigate the in vivo distribution of cytokine induced killer （CIK） cell after intravenous injection in animals. Methods CIK cell was labeled with ^99Tc^m. Forty mice were equally divided into 8 groups. These mice were killed at 3,15,30 min and 1,3,6, 24, 48 h after intravenous ^99Tc^m-CIK cell injection. The radioactivity of blood, heart, lung, liver, spleen, kidney, brain, bone and muscle were measured. Three New Zealand rabbits were scanned by dynamic SPECT imaging after intravenous injection of 37 MBq ^99Tc^m-CIK cell. Serial images over whole body were acquired from immediately after injection to 48 h later. Results The radiochemical purity of ^99Tc^m-CIK cell was over 95% and remained stable within 6 h at room temperature. The clearance of ^99Tc^m-CIK cell in blood was fast. High uptake of ^99Tc^m-CIK cell was noted in lung, liver, spleen and kidney. Little uptake was found in heart, brain, bone and muscle. Dynamic SPECT imaging showed that ^99Tc^m-CIK cell accumulated in lungs early after injection and cleared thereafter. Then the uptake in liver increased and remained there after 2 d. There was little uptake in heart, brain, bone and muscle. Conclusions ^99Tc^m-CIK cell clears fast in the blood after injection. Within 1 h, the tracer mainly accumulates in lungs. Then the uptake is mostly in liver and spleen.

关 键 词：杀伤细胞 细胞活素类 锝 同位素标记 药代动力学 动物 实验 

分 类 号：R686[医药卫生—骨科学]