缺血后处理上调内源性抗氧化物酶活性诱导兔脊髓保护效应  

Ischemic postconditioning induces neuroprotection via up-regulation of endogenous antioxidant enzyme activities： experiment with rabbits

作　　者：宋文英[1] 董海龙[1] 陈琼[1] 路志红[1] 王慧[2] 孟静茹[2] 熊利泽[1] 

机构地区：[1]第四军医大学西京医院麻醉科,西安710032 [2]第四军医大学西京医院基础部药学系药理教研室,西安710032

出　　处：《中华医学杂志》2008年第33期2355-2359,共5页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30471664）

摘　　要：目的观察缺血后处理是否通过增加再灌注期间内源性抗氧化物酶的活性以减轻兔脊髓缺血一再灌注损伤。方法雄性新西兰大白兔78只随机分为3组：假手术组（Sham组，n=18）：操作同I／R组，但不阻闭腹主动脉；缺血一再灌注组（I／R组，n=30）：阻闭腹主动脉20min后再灌注；缺血后处理组（PostC组，n=30）：阻闭腹主动脉20min，再灌注即刻行30s再灌注／30s缺血，3个循环，其他同I／R组。在再灌注的30min、1、3、6、24和48h分别取材，采用分光光度法行脊髓抗氧化物酶活性及丙二醛（MDA）含量测定（各时间点n=5，Sham组n=3）。再灌注6、24和48h在取材前分别行后肢运动功能评分。结果①再灌注6、24和48h时PostC组后肢运动功能评分显著高于I／R组（均P〈0．05）。②PostC组脊髓组织中超氧化物歧化酶（SOD）及过氧化氢酶（CAT）活性在再灌注早期（30min～6h）显著高于I／R组（均P〈0．05）。PostC组各时间点脊髓组织谷胱甘肽过氧化物酶（GSH-px）活性与I／R组相比，差异无统计学意义。③PostC组脊髓组织MDA含量在再灌注24h和48h明显低于I／R组（P〈0．01）。结论缺血后处理可通过上调脊髓SOD及CAT活性，减轻脊髓缺血再灌注损伤，产生保护效应。Objective To test the hypothesis that spinal cord protection induced by ischemic postconditioning is mediated by an increase of endogenous antioxidant enzyme activities during reperfusion phase in spinal cord. Methods Seventy-eight male New Zealand rabbits were randomly divided into 3 groups： Sham group （n = 18） undergoing sham operation without aortic occlusion; ischemia/reperfusion （I/ R） group （n = 30） undergoing occlusion of the infrarenal abdominal aorta for 20 min, followed by reperfusion ; and postconditioning （PostC） group （ n = 30） undergoing occlusion of the infrarenal abdominal aorta for 20 min followed by 3 cycles of 30 s reperfusion/30 s ischemia just at the onset of reperfusion. 30 min, and 1, 3, 6, 24, and 48 h after reperfusion 5 rabbits from each group （ and 3 from the Sham group） were killed with their spinal cords taken out, and spectrophotometric method was used to determine the antioxidant enzyme activity and malondialdehyde （MDA） content 6, 24, and 48 h after reperfusion motor function scoring of the hind limbs was conducted. Results ① The motor function scores of the PostC group were significantly higher than those of the I/R group 6, 24, and 48 h after reperfusion （ all P 〈 0. 05）. ② The activities of superoxide dismutase （SOD） and catalase （CAT） in the spinal cord tissue of the PostC group 30 min-6 h after reperfusion were all significantly higher than those of the I/R group（ all P 〈0. 05 ）. There were no significant differences in the activity of glutathione peroxidase （GSH-px） at different time points between the PostC and I/R groups. ③ The MDA levels 24 h and 48 h after reperfusion of the PostC group were both significantly lower than that of the I/R group （ both P 〈 0. 01 ）. Conclusion Ischemic postconditioning shows effect against spinal cord ischemic-reperfusion injury mediated, at least partially, by up-regulating the activities of SOD and CAT in spinal cord during early reoerfusion phase.

关 键 词：再灌注损伤 脊髓 缺血后处理 抗氧化物酶 

分 类 号：R686[医药卫生—骨科学]