猪链球菌35个血清型标准抗血清的制备及应用  

Preparation and Application of Streptococcus suis Antisera against 35 serotypes

作　　者：姜艳华[1] 孙乾[1] 王楷宬[2] 黄保续[2] 辛长卫 姜平[1] 范伟兴[2] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]中国动物卫生与流行病学中心 [3]河南省博爱县职业中专,454492

出　　处：《中国动物检疫》2008年第9期19-21,24,共4页China Animal Health Inspection

基　　金：农业部"948"项目资助(2006-G57)

摘　　要：用猪链球菌35个血清型的标准菌株免疫新西兰白兔制备标准抗血清,经试管凝集测定抗血清的效价均在1:32以上,吸附处理后抗血清具有良好的特异性和敏感性。对12株猪链球菌的试验结果表明:抗血清只与相同血清型的菌株出现凝集,可以区分出PCR方法无法区分的1型和14型以及2型和1/2型,并在国内首次鉴定出猪链球菌13型。Antisera against the 35 serotypes＇ reference strains of Streptococcus suis were raised in rabbits, the results of agglutination tests show the titers of all the antisera are above 1：32, Cross-absorption can avoid the cross-reaction and increase the sensitivity and specificity. The test results of 12 isolated strains show the antisera can distinguish the serotype 1 and 14,2 and 1/2, which cannot be distinguish by PCR, and serotype 13 was detected and identified in China for the first time.

关 键 词：猪链球菌 抗血清 制备 应用 

分 类 号：S854.43[农业科学—临床兽医学]