微流控芯片测定滴鼻液中的盐酸萘甲唑啉  

Determination of naphazoline hydrochloride in naphazoline hydrochloride nose drops by microfluidic chip

作　　者：吴小林[1] 柯木养[2] 刘翠[2] 李偶连[2] 陈缵光[2] 

机构地区：[1]中山大学附属第三医院,广州510630 [2]中山大学药学院,广州510089

出　　处：《分析试验室》2008年第9期76-78,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20575080,20727006)项目资助

摘　　要：建立了微流控芯片非接触电导检测法测定盐酸萘甲唑啉的分析方法。探讨了缓冲液种类、浓度，添加剂种类、浓度以及分离电压等因素对分离检测的影响。优化选择20mmol／L H3BO3＋10mmol／L Tris（pH7．5）缓；中溶液，2mmol／L β-CD添加剂．分离电压2．70kV时，3min内可实现盐酸萘甲唑啉的快速分离检测。在优化条件下，盐酸萘甲唑啉的线性范围为20～1．0×10^3μg／mL（r=0．995），检出限为5．0μg／mL（S／N=3），RSD为2．5％，加标回收率为98．3％～101％。A method for the determination of naphazoline hydrochloride in naphazoline hydrochloride Nose Drops by mierofluidie chip with eontaetless conductivity detection was established in this paper. The eleetrophoretie parameters, such as the variety and concentration of buffer solution and additive, separation voltage ete, were researched. Naphazoline hydrochloride was separated and detected in the buffer solution of 20 mmol/L H3BO3 ＋ 10 mmol/L Tris （pH 7.5） with 2 mmol/L β-CD at a separation voltage of 2.7 kV within 3 min. The linear dependence of the concentration of naphazoline hydroehloride ranged from 20 μg/mL to 1.0 × 10^3 μg/mL （ r = 0. 995）, the limit of detection （LOD） reached 5.0 μg/mL （S/N= 3）, the RSD was 2.5% and the recoveries were 98.3% - 101%. The method was effective, simple and sensitive, and it could be used in the quality control of these products.

关 键 词：微流控芯片 微全分析系统 非接触电导检测 盐酸萘甲唑啉 滴鼻液 

分 类 号：O652.9[理学—分析化学]