分光光度法测定黄花菜中总黄酮  

Spectrophotometric determination of total flavonoids in daylily

作　　者：赵二劳[1] 段晋峰[1] 

机构地区：[1]忻州师范学院化学系,忻州034000

出　　处：《分析试验室》2008年第9期94-96,共3页Chinese Journal of Analysis Laboratory

基　　金：忻州师范学院科研(200699)项目资助

摘　　要：建立了一种黄花菜中总黄酮的Al（NO3）3显色分光光度测定方法。研究表明：以芦丁为标样，在一定条件下，芦丁的浓度与吸光度呈线性关系，回归方程为A=0．0121c＋0．0014，R^2=0．9999，方法的回收率为98．1％～102．3％，相对标准偏差（RSD）为2．1％，测得黄花菜中总黄酮量为3．71mg／g。A speetrophotometrie method for the determination of total flavonoids in daylily was established. Under optimum conditions, a good linear relationship between concentration of rutin and its absorbanee was obtained. Regression equation is A = 0.0121 c ＋ 0.0014, correlation coefficient R^2 = 0.9999. The recoveries and RSD are 98.1% - 102% and 2.1%, respectively. The total flavonoids in daylily are 3.17 mg/g.

关 键 词：黄花菜 总黄酮 分光光度法 Al(NO3)3 

分 类 号：O557.3[理学—热学与物质分子运动论]