绵羊鸟苷素基因的克隆与序列分析  

Molecular cloning and sequence analysis of ovine guanylin

作　　者：乔新安[1] 王艳玲[1] 杨国宇[1] 朱彦彩[2] 范沛[1] 都军霞[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002 [2]河南科技学院细胞工程重点实验室,河南新乡453003

出　　处：《甘肃农业大学学报》2008年第4期23-26,共4页Journal of Gansu Agricultural University

基　　金：河南省重点科技攻关项目(编号:0523010500)资助

摘　　要：基于电子延伸序列．本试验克隆并分析了绵羊鸟苷素基因．从绵羊十二指肠黏膜组织中提取总RNA·利用设计的引物进行RT-PCR扩增，PCR产物与pMD19-T载体连接后转化到E．coli JM109感受态细胞，检测阳性克隆并测序．结果表明：克隆的绵羊鸟苷素基因长341bp，编码109个氨基酸．与人、豚鼠、小鼠和牛的同源性分别为77％、75％、47％和94％．推测的氨基酸序列信号肽为l～16aa．整个氨基酸构成一个模域．编码鸟苷素前体蛋白．The coding sequence of ovine guanylin was cloned and analyzed based on the in silico sequence information. Total RNA was extracted from mucosal tissue of ovine duodenum and mRNA sequence was amplified by RT-PCR with designed primers. The PCR products were ligated into the pMD19-T vector,and then transformed into competent E. coli JM109. The sequence was analyzed to identify the recombinant plasmid. Sequence analysis indicated that the guanylin gene was 341 bp in length and the deduced amino acids was 109. Homology analysis showed that the sequence shared 77 %,75 % ,74 % and 94 0/4 homology with those of human, guinea pig, mouse and cattle. The signal peptide of deduced amino acid sequence is 1-16 aa and the omain is formed by the amino acid sequence coding precursor protein of guanylin.

关 键 词：绵羊 鸟苷素 克隆 序列分析 

分 类 号：S826.2[农业科学—畜牧学]