苍耳素Ⅲ-1的分离纯化及鉴定  

Isolation and identification of extract Ⅲ-1 from Xanthium sibiricum

作　　者：张帆[1] 杨顺义[1] 张新虎[1] 沈慧敏[1] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2008年第4期77-81,共5页Journal of Gansu Agricultural University

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2004-08);甘肃省教育厅基金项目(032-06)

摘　　要：以黄瓜枯萎病菌为供试菌，采用生物活性示踪法，对苍耳丙酮提取物Ⅲ-1的抑菌成分进行分离．结果表明：苍耳丙酮提取液Ⅲ-1对黄瓜枯萎病菌有抑制作用的有效成分主要集中在2～7、9～11和19、21等10个流份中，其中流份5的抑制率达到51．00％；通过薄层层析分离和GC-MS联用仪鉴定，发现苍耳素Ⅲ-1的主要抑菌成分有呋喃甲醛、5-羟甲基糠醛、4-羟基-4-甲基-2-戊酮等．The antifungal activity of the extract Ⅲ-1 from Xanthium sibiricumwas was tested with Fusarium oxysporium. The antifungal composition of the acetone extraction Ⅲ-1 was isolated primarily with column chromatography. The results showed that the antifungal activity appeared in fractions of 2 to 7,9 to 11 ,and 19,and 21. The fifth fraction had the highest growth inhibition rate with 51.00 %. By thin layer chromatography （TLC） isolation and gas chromatography and mass spectroscopy （GC-MS） identification antifungal,the primary antifungal ingredients were 2-furancarboxalde, 5-（hydroxymethyl）-2-furaldehyde, diacetone alcohol.

关 键 词：苍耳素Ⅲ-1 抑菌成分 分离纯化 鉴定 

分 类 号：S482.2[农业科学—农药学]