巴彦淖尔地区汉坦病毒的分子流行病学调查  

The Molecular Epidemiological Investigaton of Hantavirus in Bayannaoer Region

作　　者：杨鹏飞[1] 马超[1] 高娜[1] 张永振[2] 祝建波[1] 

机构地区：[1]新疆石河子大学生命科学学院,新疆石河子832003 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《西南师范大学学报（自然科学版）》2008年第3期109-112,共4页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：目的:对巴彦淖尔地区进行汉坦病毒的分子流行病学调查.方法:采用间接免疫荧光法(IFA)对在野外捕获的鼠肺样品进行初筛,用逆转录-聚合酶链式反应(RT-PCR)扩增IFA阳性标本中汉坦病毒的S基因片段,以S基因片段(620-999 nt)的核苷酸序列构建系统发生树并进行分子进化分析.结果:捕获的200只中有褐家鼠141只(70.5%),黑线仓鼠40只(20.0%),小家鼠19只(9.5%);IFA检出6份阳性标本,均来自褐家鼠,其S基因均为S1亚型.结论:巴彦淖尔地区褐家鼠携带S1亚型汉坦病毒.Objective： to explore the molecular epidemiology of hantavirus carried by hosts in the city of Bayannaoer in Inner Mongolia Autonomous Region. Methods： collecting rodents in the Bayannaoer areas, rats lungs were detected by indirect immunofluorescence assay（IFA）. The hantaviral S gene of positive samples were covered by reverse transcriptase-polymerase chain reaction（RT-PCR）, and then phylogenetic tree was constructed based on S segments（620-999 nt）. Results： a total of 200 rodents were trapped, and hantavirus antigens were identified in 6 out of these lung samples. Phylogenetic analysis of these S segment sequences indicated that all viruses belonged to Seoul virus （SEOV）. Conclusion： the main genotype of hantavirus carried by Rattus norvegicus in Bayannaoer Region were S1 subtype of SEOV.

关 键 词：汉坦病毒 RT—PCR 系统发生分析 基因分型 

分 类 号：Q939.4[生物学—微生物学]