采用量子点标记的寡核苷酸探针检测ApoEε4等位基因  

Detection of ApoEε4 Allele by Quantum Dots Labeled Oligonucleotide Probe

作　　者：杨华静[1] 徐鹏[2] 王坚苗[3] 夏星[4] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]华中科技大学同济医学院附属协和医院心血管外科,武汉430022 [3]华中科技大学同济医学院附属同济医院呼吸内科,武汉430030 [4]华中科技大学物理系,武汉430074

出　　处：《华中科技大学学报（医学版）》2008年第4期493-495,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家高技术研究发展计划(863计划)资助项目(2003BA310A28)

摘　　要：目的探讨新型纳米荧光材料量子点标记的寡核苷酸探针在确定基因型中的应用价值。方法采用以CdSe为核心的量子点与ApoEε4基因的等位基因特异性寡核苷酸反应合成探针,对ApoEε4-PT7-PL质粒和正常人标本进行Southern blot检测,并与地高辛标记方法作相应比较。结果量子点与地高辛标记显影结果完全一致。结论量子点在Southern blot检测成像中表现出优良特性,可望替代传统标记物。Objective To investigate the applied value of the novel fluorescent nanocrystal-quantum dots （QDs） labeled oligonucleotide probe in genotype assay. Methods QDs were successfully conjugated with idiosyncratic oligonucleotide from ApoEε4 allele to synthesize probe. The plasmid and normal people samples were detected with QDs-labeled or dig-labeled probe by Southern blot, and image difference and photostability were compared. Results QDs-labeled and dig-labeled probes were identical in Southern blot imaging. Conclusion QDs exhibit extremely desirable properties for Southern blot imaging, and they may substitute for the existing markers.

关 键 词：量子点 ApoEε4等位基因 寡核苷酸探针 Southern印迹法 

分 类 号：R349.81[医药卫生—基础医学]