氧氟沙星镍配合物的合成、红外光谱、热分析与抗肿瘤活性  

Synthesis,infrared spectra,thermogravimetric analysis,antibacterial and antitumor activities of Ni(Ⅱ) ofloxacin complex

作　　者：陈威[1] 雷群芳[1] 王国平[1] 

机构地区：[1]浙江大学化学系,浙江杭州310027

出　　处：《浙江大学学报（理学版）》2008年第5期553-556,共4页Journal of Zhejiang University（Science Edition）

基　　金：浙江省医药卫生科学研究基金资助项目(2005A071)

摘　　要：合成了以氧氟沙星为配体的镍(Ⅱ)配合物Ni(ofo)2·4H2O,通过红外光谱和热分析等方法对配合物的结构进行了表征,推测了配合物的可能结构,并利用液体稀释法测定了药物配体和配合物对两种革兰氏阳性菌和两种革兰氏阴性菌的体外抑制活性.结果表明配合物与药物配体的抗菌活性和抗菌谱相同.采用MTT比色法测定了配体及配合物对HL-60(人急性早幼粒白血病)细胞的抑制作用,采用SRB蛋白染色法测定了配体及配合物对BEL-7402(人肝细胞性肝癌)细胞的抑制作用.结果表明Ni(ofo)2·4H2O对BEL-7402细胞没有抑制作用,对HL-60细胞在较高浓度时有一定的抑制作用.Ofloxacin （ofox） is able to interact with Ni（ Ⅱ ） salt to form coordination compound with the formula Ni （ofox）2 · 4H2O. Bonding takes place through one of the oxygen atoms of the carboxylate group （acting as a monodentate） and the oxygen atom of the ketonic group. The IR bands of earboxylic and ketonic group at 1713 and 1622 cm^-1, respectively, shift to 1612 and 1571 cm^-1 in the coordination compound. Thermogravimetric （TG） and its differential （DTG） for ofox and the coordination compound were carried out and explained in detail. Both ligand and coordination compound were assayed against gram-positive and gram-negative bacteria by in vitro doubling dilutions method, the coordination compound showed the same minimal inhibitory concentration （MIC） as the corresponding ligand against S. Aureus, M. Lutens, E. Coliand P. Aeruginosa. The inhibitory effect of the ligand and coordina tion compound on leukemia HL-60 cell line was measured by using MTT （Methyl-Thiazol-Tetrozolium） assay method and that on liver cancer BEL-7402 cell line was measured by SRB （Sulphurhodamin B） method. The results indicared that the coordination compound has inhibitory effect on HL-60 cell line.

关 键 词：配合物 氧氟沙星 红外光谱 热分析 抗菌活性 抗肿瘤活性 

分 类 号：O614[理学—无机化学]