沙眼衣原体抑制etoposide诱导的HeLa229细胞凋亡的研究  

Study on chlamydia trachomatis inhibition of apoptosis induced by etoposide in Hela229 cell

作　　者：杨志英[1] 张贤建[1] 欧录明[1] 余平[2] 

机构地区：[1]湘南学院基础医学课部,湖南郴州423000 [2]中南大学免疫学系,湖南长沙410078

出　　处：《中国现代医学杂志》2008年第16期2336-2339,2343,共5页China Journal of Modern Medicine

基　　金：湖南省教育厅基金项目(No:06C787)

摘　　要：目的探讨沙眼衣原体D血清型感染对宿主细胞凋亡的影响。方法用凋亡诱导剂依托泊苷(e-toposide)作用于沙眼衣原体(D血清型)感染的和未感染的Hela229细胞,Hoechst33258染色后荧光显微镜观察核浓缩和凋亡小体,流式细胞仪检测细胞周期和凋亡率。结果未感染CT的Hela229细胞经etoposide作用后,用荧光显微镜观察到明显的核浓缩和凋亡小体;流式细胞仪检测的凋亡率为90.64%,与未经诱导剂作用的Hela229细胞比较差异有显著性(P<0.05)。而CT感染24h的Hela229细胞,经etoposide作用后未观察到核浓缩和凋亡小体;流式细胞仪检测的凋亡率为11.50%,与未感染CT的Hela229细胞诱导后的凋亡率比较差异有显著性(P<0.05);而与未经诱导的CT感染Hela229细胞比差异较无显著性(P>0.05)。结论沙眼衣原体感染Hela229细胞后能抑制etoposide诱导的细胞凋亡。[Objective] To explore the effect of C. trachomatis （serovar D）infection on apoptosis of host cell. [Methods] Induced by etoposide, Detection of ceil apoptosis： Nuclear condensation and apoptotic bodies were observed by fluorescence microscope after Hoechst 33258 stain. Apoptotic index were analyzed by flow cytometer assay （FCM）. [Results] Induced by etoposide, uninfected Hela229 cells showed nuclear condensation and apoptosis bodies. FCM showed that the apoptotic rate was 90.64% in uninfected Hela229 ceils. There were significant difference compared with normal group （P〈0.05） While Hela229 ceils infected 24 hours with C. trachomatis was induced by etoposid, there were no nuclear condensation and apoptosis bodies. Its apoptotic rate was 11.50%. There were significant difference compared with uninfected Hela229 cells induced by etoposide （P 〈0.05） and no significant difference with infected-Heia229 ceils （P 〉0,05）. [Conclusions] C. trachomatis infection can inhibit apoptosis of infected Hela229 cell induced by etoposide.

关 键 词：沙眼衣原体(D血清型) HELA229细胞 凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]