樱桃带化病组织中植物菌原体16SrDNAPCR扩增及其产物RFLP分析  

PCR Amplification of 16 S rDNA of Phytoplasma Associated with Cherry Fasciated Disease and RFLP Analysis

作　　者：李横虹[1,2,3] 邱并生[1,2,3] 史春霖[1,2,3] 金开璇[1,2,3] 周琦 黄习军 

机构地区：[1]中国科学院微生物研究所 [2]中国林业科学研究院森林保护研究所 [3]中华人民共和国首都机场动植物检疫局

出　　处：《林业科学研究》1997年第5期478-481,共4页Forest Research

基　　金：国家自然科学基金

摘　　要：樱桃带化病是从以色列引种樱桃园中发现的新病害。本文报道了按照检测植物菌原体（ｐｈｙｔｏｐｌａｓｍａ）的方法提取ＤＮＡ，扩增患病植株中植物菌原体的１６ＳｒＤＮＡ片段，证明樱桃带化病中有植物菌原体存在，并对此扩增片段进行限制性酶切片段长度多态性（ＲＦＬＰ）分析。根据ＲＦＬＰ分析结果，参照Ｌｅｅ，Ｉ．Ｍ．等的文献资料，对本次樱桃带化病病原进行鉴定与分类，初步定为Ⅶ类。Cherry fasciated disease is a disease which was found in the cherry sapling imported from Israel.DNA amplification by PCR was used to detect the phytoplasma associated with cherry fasciated disease.The 16 S rDNA sequence amplified was analyzed by restriction endonuclease digestion.The RFLP pattern(sum of results analyzed by 7 restriction enzymes)was compared with the results of other phytoplasma previously described by Lee,I.M.et al.We propose that cherry fasciated disease should belong to Group Ⅶ.

关 键 词：樱桃 带化病 植物菌原体 RFLP 分析 

分 类 号：S436.629[农业科学—农业昆虫与害虫防治]