斜纹夜蛾核型多角体病毒不同分离株基因序列的同源性分析  

Homologous analysis of gene sequences in Spodoptera litura multinucleocapsid nucleopolyhedrovirus isolates

作　　者：刘艳荷[1] 方继朝[1] 

机构地区：[1]江苏省农业科学院植物保护研究所

出　　处：《昆虫学报》2008年第8期817-823,共7页Acta Entomologica Sinica

基　　金：中国博士后科学基金项目(20060390946);江苏省博士后基金;江苏省农业科技创新资金项目[cx(07)606]

摘　　要：研究SpltMNPV不同分离株及SpltMNPV分离株与SpliNPV间基因序列的同源性,为SpltMNPV分离株的利用提供理论基础。根据已发表的斜纹夜蛾核型多角体病毒(SpltMNPV)中国株(Zh)基因组全序列(AF527603)和海灰翅夜蛾核型多角体病毒(SpliNPV)NotI-D片段序列(AF527603)设计引物,PCR方法扩增得到SpltMNPV日本福冈株(Fu)、埃及株(Eg)和小笠原株(Og)的ORF39～ORF42和ORF119～ORF124编码区全序列。SpltMNPV不同分离株及SpltMNPV分离株与SpliNPV间基因序列的相似性比较,Zh株和Og株,Eg株、Fu株和SpliNPV的相似性高,而Zh株和Eg株、Fu株或SpliNPV,Og株和Eg株、Fu株或SpliNPV的相似性都比较低。亦即SpltMNPV3种基因型,B型和C型的同源性高,A型与B型或C型的同源性比较低,但A型与SpliNPV的同源性高;同一基因型内不同分离株(Eg株和Fu株)的同源性高。ETG分子进化分析表明Eg株、Fu株和SpliNPV处于一个分支,而Eg株、Fu株和SpliNPV与Zh株和Og株则处于不同的分支。因此推断Eg株和Fu株为SpliNPV的分离株,而Og株为SpltMNPV的分离株。In order to provide the theory base for the utilizing of SpltMNPV isolates,the homologous analysis of gene sequences among Spodoptera litura multinucleocapsid nucleopolyhedrovirus（SpltMNPV） isolates or between SpltMNPV and S.littoralis nucleopolyhedrovirus（SpliNPV） were studied.According to the complete genome sequence（AF527603） of SpltMNPV and the sequence（AF527603） of SpliNPV Not I-D fragment deposited in GenBank,five pairs of primers were designed and used.ORF39-ORF42 and ORF119-ORF124 of SpltMNPV three isolates,i.e.,Fukuoka（Fu）,Egypt（Eg） and Ogasawara（Og）,were amplified by PCR.The comparison of ORF39-ORF42 and ORF119-ORF124 nucleotide and amino acid sequences of SpltMNPV isolates,i.e.,Chinese isolate（Zh）,Eg,Fu and Og,with those of SpliNPV indicated that the similarity between Zh and Og,or between Eg,Fu and SpliNPV is as high as over 99%,but the similarity between Zh and Eg,Fu,SpliNPV or between Og and Eg,Fu,SpliNPV is as low as 74%.This means that among SpltMNPV three genotypes,the homology between B and C is high,A and B or C is low,but the homology between A and SpliNPV is high.The homology of the isolates within the same genotype is high too.The neighbor-joining tree based on amino acid sequences of the ecdysteroid UDP-glucosyltransferase（EGT） in 29 nucleopolyhedroviruses and 10 granuloviruses was established.In the phylogenetic tree,EGTs of Eg and Fu clustered into one clade with SpliNPV,which belonged to a larger clade that included the other SpltMNPV isolates.Based on the sequence alignment and the phylogenetic tree of baculovirus EGTs,it is inferred that Eg and Fu are the isolates of SpliNPV,but Og is the isolate of SpltMNPV.

关 键 词：斜纹夜蛾核型多角体病毒 分离株 基因 序列分析 同源性 系统发育 

分 类 号：Q965.8[生物学—昆虫学]