一个新的葡萄抗逆转录因子VvERF2基因的In silico克隆及生物信息学分析  

In silico cloning and characterization of a novel transcription factor VvERF2 from Vitis vinifera with bioinformatics tools

作　　者：刘红锦[1] 高峰[2] 熊爱生[2] 彭日荷[2] 李贤[2] 蔡斌[2] 薛永[2] 付晓燕[2] 

机构地区：[1]江苏省农科院农产品加工研究所,南京210014 [2]上海市农业科学院生物技术研究所,上海201106

出　　处：《上海农业学报》2008年第3期10-15,共6页Acta Agriculturae Shanghai

基　　金：863项目(2006AA102117);上海市青年科技启明星跟踪计划(08QH14021);上海市优秀学科带头人计划(06XD14017)资助项目

摘　　要：ERF是植物所特有的一类重要的转录因子,广泛参与植物生长、发育以及多种生理生化反应的信号传导。本研究利用In silico克隆方法获得葡萄VvERF2基因,并利用生物信息学方法对该基因编码产物从氨基酸组成、理化性质、进化关系、二级及三级结构、功能等方面进行预测和分析。结果表明,VvERF2为亲水性蛋白,与其他物种的ERF在序列组成、结构及活性位点等方面均具有高度的一致性。ERF is an important transcription factor peculiar to plants and widely participates in plant growth, development and signal transduction of varied physiological and biochemical reactions. In this paper,a novel transcription factor VvERF2 from Vitis vinifera was cloned by in silico cloning based on the EST sequences from EST assembly of PlantGDB. And with bioinformatics tools this gene coded products were analyzed and predicted in terms of amino acid composition, physicochemical properties, secondary and tertiary structures of protein, function and so on. The results showed that VvERF2 was a hydrophilic protein and had a high consistency with other plants＇ ERF in such aspects as structure, sequence and active sites.

关 键 词：ERF In silico克隆 葡萄 转录因子 生物信息学 

分 类 号：S663.1[农业科学—果树学] Q811.4[农业科学—园艺学]