三氧化二砷对小鼠小脑氧化还原相关酶基因表达谱的影响  

Influence of arsenic trioxide on gene expression profile of oxidation reduction enzyme in the cerebellum of mice

作　　者：洪岩[1] 朴丰源[1] 王艳艳[1] 刘鹏[1] 

机构地区：[1]大连医科大学卫生学教研室,辽宁大连116044

出　　处：《毒理学杂志》2008年第4期263-265,共3页Journal of Toxicology

基　　金：国家自然科学基金(30571584;30600488);辽宁省教育厅项目(05L113)

摘　　要：目的应用基因芯片技术观察三氧化二砷(As2O3)对小鼠小脑组织氧化还原相关酶基因表达谱的影响。方法昆明种小鼠30只随机分为3组,即生理盐水对照组、低剂量组(1 mg/L As2O3染毒组)和高剂量组(4 mg/L As2O3染毒组),连续染毒60 d,断头法处死小鼠,利用基因芯片技术检测基因表达谱的变化。结果基因芯片筛选结果显示,与对照组比较,染砷组中差异表达2倍及以上的基因有18条,其中表达上调的基因有12条,表达下调的基因有6条。高剂量组与低剂量组及对照组比较,表达上调的基因有Ndufa4、Ndufa6、Gpx3、Adi1、Rrm2b,表达下调的基因有Spr、Hsd17b11、Ogfod1、Ndufab1。与对照组比较,染砷组中Cyp51、Phgdh、Dhrs4、Prdx4、Aldh1a、1810063B05Rik、Glrx表达上调,Prdx2、1110020P15 Rik表达下调。结论As2O3对小鼠小脑的氧化还原相关酶基因表达谱具有明显的影响,提示这些小脑氧化还原相关酶基因很可能是砷的神经毒作用的靶点。Objective To study influence of arsenic trioxide （As2O3 ） on gene expression profile of oxidation reduction enzyme in the cerebellum of mice by gene- chip. Methods Tirty mice were divided into 3 groups, including the 2 experimental groups （ 1 mg/ L or 4 mg/L As2O3 . ） and one controls. As2O3 was given for consecutive 60 days, followed by decollation. Detecting the alteration of gene expression profile by genechip. Results The results of geneship screening indicated that 18 genes fragments in experimental groups were shown in differential expression profile, 2 times and above with 12 upregulated genes and 6 downregulated genes. High dose group compared to low dose and control groups, upregulated genes included Ndufa4 ,Ndufa6 ,Gpx3 ,Adil and downregulated genes included Spr, Hsdl7bll ,Ogfodl ,Ndufabl. Compared to control group. Cyp51 ,Phgdh, Dhrs4,Prdx41 ,Aldhla,810063BO5Rik,Glrx were upregulated in the groups exposed to arsenic, Prdx2,1110020P15Rik were downregulated. Conclusion Evident influence of gene expression profile of oxidation reduction enzyme in the cerebellum of mice exposed to arsenic, hinting that the these genes may target of cerebellum neurotoxicity to arsenic.

关 键 词：三氧化二砷 基因芯片 小脑 氧化还原酶 差异表达基因 

分 类 号：R994.6[医药卫生—毒理学]