大蒜油对CCl_4引起的急性化学性肝损伤的预防作用  

The preventive effect of garlic oil on the acute chemical liver injury induced by carbon tetrachloride

作　　者：张翠丽[1] 曾涛[1] 王青山[1] 于丽华[1] 朱振平[1] 赵秀兰[1] 谢克勤[1] 

机构地区：[1]山东大学公共卫生学院毒理学研究所,山东济南250012

出　　处：《毒理学杂志》2008年第4期291-293,共3页Journal of Toxicology

摘　　要：目的研究大蒜油对四氯化碳(carbon tetrachloride,CCl4)引起的急性化学性肝损伤是否有预防作用。方法雄性昆明小鼠90只,随机分为阴性对照组、溶剂对照组、CCl4模型组、大蒜油低、中、高剂量组,每天灌胃1次/d,30 d后模型组和大蒜油组一次性灌胃给予CCl480 mg/kg,24 h后取血测定各组小鼠血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活力,取肝脏,称重,并计算肝脏系数,同时取肝左叶进行病理组织学检查。结果与阴性对照组相比,CCl4模型组血清ALT、AST活力明显升高,肝脏重量和肝脏系数明显增加(P<0.01)。与模型组相比,大蒜油低、中、高剂量组血清ALT、AST活力明显降低(P<0.01),并存在剂量-效应关系;大蒜油低、中、高剂量组肝脏重量和肝脏系数明显减小(P<0.01);同时大蒜油可有效减轻CCl4引起的细胞坏死、气球样变等肝细胞损伤。结论大蒜油对CCl4引起的急性化学性肝损伤有明显的预防作用。Objective To study the preventive effect of garlic oil on carbon tetrachloride （CCl4）-induced acute chemical liver injury. Methods 90 male KM mice were randomly divided into negative control group, solvent control group, CCl4 model group, garlic oil low, mid, high groups. The mice were gavaged 1 time/day. After 30 days, model group and garlic oil groups were given a single dose of CCl4 80 mg/kg （po）. The indexes including the activities of ALT and AST, liver weight, liver coefficient, and hepatic histopathology examination. Results CC}~ significantly elevated the activities of serum ALT and AST, the liver weight, and the liver coefficient （ P 〈 0.01 ）. Compared with model group, the activities of ALT and AST in serum were reduced gradually with the garlic oil dose raising （P 〈 0.01）. The liver weight, the liver coefficient in garlic oil low, mid, and high groups were decreased remarkably （P 〈 0.01 ）. Hepatic histopathology detection showed that garlic oil effectively alleviated hepatocyte injury induced by CCl4 . Conclusion Garlic oil can effectively prevent the acute chemical liver injury induced by CCl4 .

关 键 词：大蒜油 四氯化碳 肝损伤 丙氨酸转氨酶(ALT) 天冬氨酸转氨酶(AST) 

分 类 号：R994.4[医药卫生—毒理学]