慢性心力衰竭患者血清白细胞介素-12及白细胞介素-18水平及意义  

Study of the serum levels and roles of cytokines IL-12,IL-18 in patients with chronic heart failure

作　　者：毛奕[1] 夏春燕[1] 程翔[1] 姚瑞[1] 余娴[1] 谢江娇[1] 丁英俊[1] 唐婷婷[1] 廖玉华[1] 

机构地区：[1]华中科技大学协和医院心内科华中科技大学同济医学院心血管病研究所,武汉430022

出　　处：《临床心血管病杂志》2008年第7期494-496,共3页Journal of Clinical Cardiology

基　　金：国家自然科学基金资助(No:30600234);国家重点基础研究发展计划(973计划)资助(No:2007CB512000;2007CB512005)

摘　　要：目的：检测慢性心力衰竭患者（CHF）外周血白细胞介素（IL）-12和IL-18的水平并探讨其意义。方法：CHF患者72例，按照NYHA心功能分级，分为HF1组（NYHA Ⅱ～Ⅲ级），HF2组（NYHA Ⅳ级）。按照病因分类，分为缺血性心脏病（IHD）组（43例）和非缺血性心脏病（NIHD）组（29例）。选取29例健康志愿者作为正常对照组（C组）。采用双抗体夹心酶联免疫吸附法（ELISA）检测血清中IL-12和IL-18的水平，用彩色多普勒超声诊断仪测定左室舒张末期内径（LVEDd）和左室射血分数（LVEF）。结果：HF1、HF2、IHD和NIHD组IL-12的水平显著升高，分别为（34．56±19．10），（35．12±15．18），（31．18±7．80），（32．73±11．54）ng／L，与C组（28．73±13．43）ng／L比较，均差异有统计学意义（P〈0．05），IL-18的水平分别为（39．50±5．66），（46．76±12、66），（40、58±13．10），（42．53±6．16）ng／L，明显高于C组（31．82±5．79）ng／L，均差异有统计学意义（P〈0．05或P〈0．01），HF2组血清IL-18水平显著高于HF1组（P〈0．05）。与C组比较，HF1、HF2、IHD和NIHD组的LVEDd明显增大，LVEF显著降低。IL-12水平与LVEDd呈低度正相关（P〈0．01），IL-18水平与LVEDd呈中度正相关（P〈0．01），与LVEF呈中度负相关。结论：IL-12和IL-18在CHF患者血清中表达升高；IL-12的水平与患者基础病因和心功能均无明显相关性；IL-18的水平与CHF的基础病因无明显相关性，但与患者的CHF程度呈正相关。Objective：To investigate the serum levels of IL-12, IL-18 in patients with chronic heart failure, and explore the roles of the cytokines in the pathogenesis of chronic heart failure. Method： Seventy-two patients with chronic heart failure were divided into 2 groups according to the New York Heart Association （NYHA） classification system： group HF1 （ Ⅱ-Ⅲ ） and group HF2 （Ⅳ）, of which 43 patients with ischemic heart disease （group IHD）, 29 with non-ischemic heart disease （group NIHD）. Twenty-nine healthy donors were enrolled as group C. The serum levels of IL-12 and IL-18 in the patients and donors were detected by ELISA. Result： The results showed that：①The level of IL-12 in group IHD and NIHD is （31. 18±7.80）ng/L,（32.73± 11.54）ng/L; were significantly higher than group C （28.73±13.43）ng/L （P〈0.05）, so were in group HF1 and HF2 （HF1[34.56 ±19.10]ng/L; HF2 [35.12 ± 15.18] ng/L） compared with group C （P 〈 0.05）. But there was no significant difference between group HF1 and HF2, so were in group IHD and NIHD; ②The level of IL-18 is significantly higher in group IHD （40, 58±13, 10）ng/L and group NIHD （42.53±6.16）ng/L compared with group C （31.82 ±5.79）ng/L（ P〈0.05）, but there was no obvious difference between group IHD and NIHD while there was significant difference between group HF1 and HF2. Conclusion：The serum levels of IL-12 and IL-18 were elevated in chronic heart failure patients. The level of IL-18 was related with the heart function. Both IL-12 and IL-18 might participate in the development of heart failure.

关 键 词：心力衰竭 细胞因子 白细胞介素12 白细胞介素18 

分 类 号：R541.6[医药卫生—心血管疾病]