血管内超声评价脂联素对动脉粥样硬化斑块的影响  

Effects of adiponectin on atherosclerotic plaque evaluated by intravascular ultrasound in rabbits

作　　者：李长江[1] 张梅[1] 张运[1] 孙惠文[1] 陈良[1] 荣瑗瑗[1] 王荣[1] 

机构地区：[1]教育部和卫生部心血管重构和功能研究重点实验室山东大学齐鲁医院心内科,济南250012

出　　处：《中华超声影像学杂志》2008年第8期716-719,共4页Chinese Journal of Ultrasonography

基　　金：国家重点基础研究发展计划（973重大课题2006CB503803）;国家高技术研究发展计划（863计划,2007AA02Z448）

摘　　要：目的利用血管内超声技术观察脂联素对动脉粥样硬化斑块的影响。方法构建携带脂联素基因的腺病毒表达载体（Ad-APN），将其转染到已形成粥样硬化斑块的新西兰大白兔腹主动脉内膜，通过血管内超声和病理学测量斑块的面积。结果新西兰大白兔腹主动脉局部转染Ad-APN后，斑块面积与对照组相比缩小36．39％（P〈0．01），管腔面积狭窄率减小23．37％（P〈0．05）；Ad-APN组与转染前比较斑块面积缩小37．50％（P〈0．01），管腔面积狭窄率减小33．15％（P〈0．01）。油红O染色结果相似，局部转染Ad-APN使斑块面积缩小30．70％（P〈0．05），斑块最大厚度减小20．83％（P〈0．05）。结论血管内超声技术能在体准确评价脂联素对动脉粥样硬化斑块的作用。Objective To investigate the effects of adiponectin on the development of atherosclerosis. Methods The in vivo role of adiponectin on the development of atherosclerosis in rabbits was investigated mainly using adiponectin-producing adenovirus （Ad-APN） and intravascular ultrasound （IVUS）. On day 14 after Ad-APN local transfer to the intima of abdominal aortas, abdominal IVUS images were recorded and then rabbits were sacrificed. Abdominal aortas were collected and performed histochemical analysis. Results Ultrasonography revealed a significantly reduced atherosclerotic area, in abdominal aortas of rabbits infected through intima with Ad-APN,by 36.39% compared with the area in adenovirus expressing 13 galactosidase gene （Ad-βgal） treated rabbits （ P 〈0.01）,and by 37.50% compared with that before treatment （ P 〈0.01）. The lumen area stenosis was also reduced by 23.37% （ P 〈 0.05） and 33.15% （ P 〈 0.01 ）, respectively. In rabbits with Ad-APN infection, the atherosclerotic plaque area as seen on Oil Red O staining was reduced significantly, by 30.70%（ P〈 0.05） and the greatest thickness of plaque was reduced,by 20.83% （ P 〈0.05） as compared with those in Ad- βgal-treated rabbits, respectively. Conclusions Intravascular ultrasound analysis is a valuable method in the in vivo study about the effects of adiponectin on the development of atherosclerosis

关 键 词：腔内超声检查 颈动脉狭窄 动脉粥样硬化 脂联素 

分 类 号：R686[医药卫生—骨科学]