多巴胺D1类受体对血管平滑肌细胞胰岛素样生长因子1受体表达的影响  

The Effect of D_1 Dopamine Receptor on IGF-1 Receptor Expression in Vascular Smooth Muscle Cells

作　　者：韩愈[1] 于长青[1] 黄河飞[1] 何多芬[1] 石伟彬[1] 傅春江[1] 杨成明[1] 王旭开[1] 黄岚[2] 周林[1] 曾春雨[1] 

机构地区：[1]第三军医大学大坪医院(野战外科研究所)心内科心血管病研究室,重庆400042 [2]第三军医大学新桥医院心内科,重庆400037

出　　处：《中华高血压杂志》2008年第8期720-723,共4页Chinese Journal of Hypertension

基　　金：国家自然基金资助项目(30470728、30672199);国家科技部973前期项目(2008CB517308)

摘　　要：目的观察多巴胺 D_1类受体对血管平滑肌细胞胰岛素样生长因子1(IGF-1)受体的影响。方法以A10细胞为研究对象,免疫印迹观察刺激 D_1类受体对 IGF-1受体表达的影响,并初步探讨 D_1类受体对 IGF-1受体影响的机制。结果刺激 D_1类受体可降低 IGF-1受体的表达,D_1类受体阻断剂可完全阻断 D_1类受体激动剂Fenoldopam 对 IGF-1受体的影响,免疫印迹显示运用蛋白激酶 C(PKC)和促分裂原活化蛋白激酶(MAPK)阻断剂后,IGF-1受体表达恢复,提示 PKC 和 MAPK 途径可能参与了 D_1类受体对血管平滑肌细胞 IGF-1受体的影响过程,在 Wistar-Kyoto(WKY)大鼠和自发性高血压大鼠(SHR)血管平滑肌细胞中 D_1类受体激动剂 Fenoldopam 对IGF-1受体表达具有抑制作用,但该抑制作用在 SHR 细胞明显低于 WKY 细胞。结论 D_1类受体对血管平滑肌细胞 IGF-1受体表达具有抑制作用,该作用可能通过 PKC 和 MAPK 途径发挥一系列生理效应。Objective To investigate the effect of D1-like dopamine receptor on insulin growth factor-1 （IGF- 1） receptor in vascular smooth muscle cells.. Methods The A10 cells were used in this study, the receptor expression was determined by immunoblotting. Results Stimulation of D1-like receptor inhibited the IGF-1 receptor expression, which was blocked by D1-like receptor antagonist. In the presence of protein kinase C （PKC） inhibitor or mitogenic activated protein kinase （MAPK） inhibitor, the inhibitory effect of D1-like receptor on IGF-1 receptor expression was abolished, indicating that PKC and MAPK were involved into the signal pathway. Stimulation of D1- like receptor expression in primary vascular smooth muscle cells from Wistar-Kyoto （WKY） rats and spontaneously hypertensive rats （SHRs） with fenoldopam inhibited IGF-1 receptor expression. However, the inhibitory degree was greater in WKY cells than in SHR cells. Conclusion Stimulation of D1-like receptor inhibits the IGF-1 receptor expression in vascular smooth muscle cells; PKC and MAPK are involved into the signal pathway.

关 键 词：D1类多巴胺受体 胰岛素样生长因子1受体 血管平滑肌细胞 

分 类 号：R544.1[医药卫生—心血管疾病]