CD151基因转移对大鼠缺血心肌VEGF表达影响的实验研究  

The effect of CD151 gene delivery on the expression of VEGF in rat myocardial infarction model

作　　者：郑振中[1] 梁静[1] 王梦洪[1] 郑泽琪[1] 彭景添[1] 魏云锋[1] 刘正湘[2] 

机构地区：[1]南昌大学第一附属医院心血管内科,江西南昌330006 [2]华中科技大学同济医学院附属同济医院心血管内科

出　　处：《中国老年学杂志》2008年第16期1565-1568,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金(30570728);湖北省卫生厅科研基金重点项目(JX2A04)

摘　　要：目的通过将携带人CD151基因的重组腺病毒相关病毒(rAAV)载体注入缺血心肌,观察CD151对缺血心肌VEGF表达的影响。方法制作大鼠急性心肌梗死模型,将rAAV-CD151注入缺血心肌。4w后取注射部位心肌,用逆转录聚合酶链式反应(RT-PCR)检测外源性CD151 mRNA的表达、Western印迹法检测CD151及VEGF的表达情况,了解高表达CD151对VEGF表达的影响。结果术后4w,CD151组心肌组织检测到外源性CD151 mRNA的表达,假手术组、对照组、GFP组未检测到外源性CD151 mRNA的表达,且CD151组心肌组织CD151蛋白表达水平高于假手术组、对照组、GFP组(均P<0.05);CD151组VEGF表达无明显增加,与对照组、GFP组比较无明显增加(均P>0.05)。结论rAAV-CD151可以有效转染大鼠心肌组织,促进缺血心肌的血管生成,改善左室功能,但对VEGF表达没有影响。Objective To investigate the effect of CD151 gene divery on VEGF in rat myocardial infarction （MI） model. Methods Rat MI model was made by ligation of anterior descending coronary artery, and rAAV-CD151 was injected into the ischemic myocardium. At 4 w after coronary artery ligation, human CD151 mRNA was detected using RT-PCR, Western blot analysis for CD151 ,VEGF were performed, Results Human CD151 mRNA was detected in rAAV-CD151 group. Compared with rAAV-GFP and control groups, CD151 gene delivery significantly increased the expression of CD151 after MI （P 〈 0. 05 ）, but did not affect the expression of VEGF（ P 〉 0. 05 ）. Conclusions CD151 could promote angiogenesis after MI in rats and improve left ventrieular function, but VEGF is not involved in the process.

关 键 词：CD151 心肌梗死 血管内皮细胞生长因子 

分 类 号：R541.4[医药卫生—心血管疾病]