胡芦巴多糖A2对大鼠肾小球系膜细胞CTGF表达的影响  

The effect of serum contained fenugreek seeds polysaccharide A2 on CTGF expression in rat mesangial cells

作　　者：石艳[1] 王路飞[2] 于晓艳[1] 苗春生[1] 李才[1] 

机构地区：[1]吉林大学药学院实验药理与毒理学教研室,吉林长春130021 [2]吉林大学第二医院眼科

出　　处：《中国老年学杂志》2008年第16期1576-1578,共3页Chinese Journal of Gerontology

基　　金：吉林省科技厅科技发展计划重大项目(20020503-4)

摘　　要：目的探讨胡芦巴多糖A2含药血清对大鼠肾小球系膜细胞细胞外基质(ECM)沉积及结缔组织生长因子(CTGF)表达的影响。方法制备胡芦巴多糖A2含药血清,体外培养大鼠肾小球系膜细胞,观察胡芦巴多糖A2血清对大鼠肾小球系膜细胞分泌纤维连接蛋白(FN)、胶原(Col)Ⅳ、TGF-β1和CTGF的影响。结果与高糖培养基组比较,A2组FN、ColⅣ含量明显下降;TGF-β1和CTGF mRNA表达明显下调。结论胡芦巴多糖A2可通过下调CTGF表达,减少高糖培养的大鼠肾小球系膜细胞FN、ColⅣ的分泌,从而抑制ECM积聚。Objective To study the effect of serum contained fenugreek seeds polysaccharide A2 on extracellular matrix （ECM） accumulation and connective tissue growth factor （CTGF） expression in rat mesangial cells. Methods Serum contained fenugreek seeds polysaccharide A2 were made,and rat mesangial cells were cultured. The effect of serum contained fenugreek seeds polysaccharide A2 on FN ,Col Ⅳ ,TGF-β1 and CTGF in rat mesangial cells were observed. Results As compared with mesangial cell media with high level of glucose group,serum contained fenugreek seeds polysaccharide A2 group decreased obviously the contents of FN, Col Ⅳ, and reduced significantly TGF-β1 and CTGF mRNA expression. Conclusions Fenugreek seeds polysaccharide A2 may inhibite ECM accumulation through decreasing CTGF expression and reducing contents of FN, Col IV in rat mesangial cells.

关 键 词：胡芦巴多糖A2 肾小球系膜细胞 结缔组织生长因子(CTGF) 

分 类 号：R285.5[医药卫生—中药学]