青阳参组培快繁技术的研究  

Studies on tissue culture and rapid propagation of Cynanchum otophyllum Schneid

作　　者：刘秀贤[1] 罗栋[1] 李正红[1] 梁宁[1] 万友名[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224

出　　处：《西南农业学报》2008年第4期1086-1088,共3页Southwest China Journal of Agricultural Sciences

基　　金：国家环保总局项目"中国重点观赏植物种质资源调查"(2004-11-07)的部分内容

摘　　要：以青阳参的成熟种子获得无菌苗,去除下胚轴和子叶作为外植体,诱导腋芽的萌发和不定芽增殖生根。结果表明:适宜的增殖培养基是MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+蔗糖3%,每个外植体的平均芽数为3.36个;适宜的诱导生根生长调节剂是ABT1号50 mg/L,生根率达到100%,平均根数为4.72条;移栽后成活率可达到90%以上。In this work, seedlings cultivated from the mature seeds of Cynartchum otophyllum were carried out without hypocotyls and cotyledons as explants the effects of adventitious bud multiplication and root induction were studied. The results showed that： The proper multiplication medium was MS ＋6-BA 2.0 mg/L ＋ NAA 0.2 mg/L ＋ sucrose 3 % ,and the average bud number of each explant was 3.36 ; 50 mg/ L ABT1 was suitable for the root induction with 100 % rooting ratio ,and the average root number was 4.72 ; The transplanting survival rate was more than 90 %.

关 键 词：青阳参 组织培养 生长调节剂 

分 类 号：S567.53[农业科学—中草药栽培]