奶牛白色念珠菌性乳腺炎的PCR检测方法的建立  

The detection of bovine mastitis caused by Candida albicans by PCR method

作　　者：刘有旺[1] 李雅林[1] 刘翠燕[2] 刘辉[2] 赵宏坤[2] 

机构地区：[1]泰山医学院基础部,山东泰安271000 [2]山东农业大学动物科技学院,山东泰安271018

出　　处：《西南农业学报》2008年第4期1116-1119,共4页Southwest China Journal of Agricultural Sciences

摘　　要：本试验针对白色念珠菌,根据白色念珠菌ITS1-ITS2部分的保守序列设计了一对特异性引物,建立了PCR检测方法。试验表明,本方法的特异性为100%,敏感性检测的最低浓度为:1.25×103CFU/mL,该方法具有快速,准确和特异的优点。Bovine mastitis caused by Candida albicans occurred frequently in recent years due to the abuse of antibiotic and the deterioration of the environment. It caused large economic loses for the dairy industry, h was important to identify the Candida albicans and control the infection rate at the farm level rapidly and exactly. A pair of specific primers were designed for Candida albicans acording to the conserved sequence of ITS1-ITS2 of the Candida albicaas. The primer specificity was 100%. The primer semitivity was1.25×10^3CFU/mL. As a result, PCR method to identify the Candida albicans in mammary gland infections of cows was developed , which was rapid, sensitive, and specific.

关 键 词：白色念珠菌 乳腺炎 PCR 检测 

分 类 号：S852.4[农业科学—基础兽医学]