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作 者:余应建[1]
机构地区:[1]重庆师范大学大学城校区建设指挥部,重庆400047
出 处:《安徽农学通报》2008年第17期200-201,234,共3页Anhui Agricultural Science Bulletin
摘 要:为了了解竹毛壳菌对化学杀菌剂的敏感程度,开发出对化学杀菌剂有抗性,生防效果稳定的遗传工程菌,采用常见化学杀菌剂进行了室内毒力测定。结果表明,该毛壳菌在不含杀菌剂的PSA培养基上能正常生长,但当PSA培养基中含0.10,0.25,0.50,1.00,2.00mg.L-1不同浓度的6种杀菌剂时,毛壳菌表现出不同程度的耐药性。毛壳菌对甲基托布津和代森锰锌更为敏感,有效抑制中浓度EC50分别为1.2572mg.L-1和1.9138mg.L-1,而对地菌灵抗性最强,有效抑制中浓度EC50为779.4711mg.L-1,敏感程度依次为甲基托布津>代森锰锌>多菌灵>三环唑>百菌克>地菌灵。In order to explicit Chaetomium sp. ity of sensitive to chemical fungicide and improve in genetically engineered microorganisms. Indoor Toxicities of six Fungicides Against Chaetomium sp. In Bamboo. The results showed that were determinated, the growth of Chaetomium sp. was normal on medium without fungicides. However, it was the power to resist those fungicides to different degrees on 6 fungicides at 5 different concentrations (0. 10, 0. 25, 0. 50, 1. 00, 2.00 mg · L^-1 ). The results indicated that Chaetomium sp. was most sensitive to thiophanate - methyl and manacozeh, and the EC50 were 1. 2572 mg · L^-1 and 1. 9138 mg· L^-1 respectively. Copper acetate provided the worst control to Chaetomium sp. , and its EC50 was 779. 4711 mg · L^-1. The fungicides sensitivities of Chaetomium sp. were ranked as: thiophanate - methyl (F) 〉 manacozeh (F) 〉earbendazim (F) 〉 tricyclazole (F) 〉 chlorothalonil (F) 〉 copper acetate (F).
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