猪链球菌2型98HAH33株脂蛋白的in silico方法鉴定  

Putative Lipoproteins of Streptococcus Suis Strain 98HAH33 Identified by in silico Genome Analysis

作　　者：孙理云[1] 王利巧[2] 毛薇[3] 

机构地区：[1]河南科技大学动物科技学院 [2]河南科技大学现代教育技术与信息中心,河南洛阳473001 [3]郑州铁路职业技术学院,河南郑州450052

出　　处：《河南科技大学学报（自然科学版）》2008年第5期61-65,共5页Journal of Henan University of Science And Technology:Natural Science

基　　金：河南科技大学博士科研启动基金项目(05-010);河南科技大学科研基金项目(2006ZY042)

摘　　要：为鉴别已公布基因组序列的SS298HAH33株的脂蛋白,以研究脂蛋白在SS2致病过程中的作用,首先利用PrositeScan和DOLOP脂蛋白预测软件预测98HAH33株的脂蛋白,采用SignalP3.0HMM,PrediSi,Phobius和LipoP-HMM分析预测的脂蛋白的信号肽,然后经"多数票决法"鉴定脂蛋白,最后利用InterProScan和BlastP服务器对鉴定的脂蛋白进行功能分析。结果鉴定出31种脂蛋白,占SS2致病株98HAH33蛋白质组的1.465%。功能分析结果表明,共有16种脂蛋白是ABC转运体的底物结合蛋白(SBP),9种SBP基因与其相应通透酶和ATPase基因组成完整ABC转运体的操纵子,参与氨基酸、金属离子、核苷、无机盐等的运输;7种具有与数据库酶域相匹配或经BlastP比对与其他酶有一致性;5种为假定的蛋白;3种脂蛋白具有其他功能。这些资料提示脂蛋白在SS2的生理和致病性方面具有重要作用。To identify putative lipoproteins of Streptococcus suis serotype 2(SS2),first,the ScanProsite feature of Prosite and DOLOP lipoprotein prediction tool is used to identify putative lipoproteins in the recently published genome of SS2 strain 98HAH33,then the identified putative lipoproteins are validated to exclude the false positive by a majority vote approach with standard tools for signal peptide verification.Finally,putative functions are attributed to individual lipoproteins by reference to the identification of conserved domains of InterPro.Homologous proteins are identified by unfiltered BlastP homology searches(including conserved domain detection).Among the 35 identified putative lipoproteins,31 are validated as lipoproteins and 4 as false positive lipoproteins.The largest functional category(51.6%)of lipoproteins may comprise substrate binding proteins of ABC transport systems.Other roles include lipoproteins that appear to participate in adhesion(YP_001199884 and YP_001200918),protein export and folding,enzymes and several species-specific proteins of unknown function.These data suggest lipoproteins make an important contribution to the physiology of SS2 and may influence its virulence.

关 键 词：猪链球菌2型 脂蛋白 生物信息学 毒力因子 

分 类 号：Q513[生物学—生物化学] S852.611[农业科学—基础兽医学]