家蚕抗核型多角体病毒病分子标记辅助新品种选育研究  

Molecular Markers-Assisted Breeding for Silkworm Resistant Variety to BmNPV

作　　者：曹锦如[1] 周文林[1] 翁宏飚[1] 叶爱红[1] 王永强[1] 

机构地区：[1]浙江省农业科学院蚕桑研究所,浙江杭州310021

出　　处：《蚕桑通报》2008年第3期19-22,共4页Bulletin of Sericulture

基　　金：浙江省科技计划重大专项项目(2006C12056)

摘　　要：利用分子标记技术开展向家蚕实用品种中导入核型多角体病毒病抗性基因的研究。实验结果发现已报道的分子标记在不同品种间表现出差异,与抗性性状并非完全相关。添毒结合标记检测,筛选出抗性品种"多化N"和敏感性的实用品种"春华"作为育种素材,并从特定品种"多化N"中获得了特异性连锁片段。通过组配近等基因系,结合添毒试验在BC3-F2代抗性个体中明显检测到该标记。Molecular markers were used in the present anti-BmNPV silkworm breeding process. The results showed that the previous report anti-BmNPV linked molecular markers alternated in different silkworm varieties and was not consistent with resistant character. Further more, one molecular marker was found linked to BmNPV resistance character in Bm NPV resistant variety ＂Duohua N＂ by genetic analysis of the near-isogenic lines BC3-F2. The virus resistant variety ＂Duohua N＂ and the susceptive commercial variety ＂Chunhua＂ were selected as the breeding material and anti-BmNPV silkworm breeding were carried out with the integration of virus feeding and molecular selection.

关 键 词：家蚕 分子标记 NPV抗性 育种 

分 类 号：S882.2[农业科学—特种经济动物饲养]