多酚氧化酶模拟酶的构建及其催化性能研究  

Synthesis and Activity of a Polyphenol Oxidase Mimic

作　　者：王秉[1] 胡智文[1] 郑海玲[1] 卢晓春[1] 温会涛[1] 万军民[1] 陈文兴[1] 

机构地区：[1]浙江理工大学先进纺织材料与制备技术教育部重点实验室,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2008年第5期539-542,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省自然科学基金项目(M503216)

摘　　要：以β-环糊精(β-CD)为模型,在其边壁上引入具有催化性质的组胺基团,得到β-环糊精的组氨酸(His)一取代衍生物(β-CD-His),并用FTIR、NMR等方法对其结构进行了表征。用β-CD-His,与Cu2+形成的配合物(Cu2+-β-CD-His)模拟多酚氧化酶催化邻苯二酚的氧化反应;反应产物用紫外-可见分光光度法进行检测,结果表明:β-CD和β-CD-His均具有一定的多酚氧化酶催化性能,β-CD-His与Cu2+络合后,催化活性明显提高。A histamine derivative of cyclodextrin（β-CD-His）is synthesised by reacting histamine, a nucleophile containing the catalytic group with the 6-tosylated cyclodextrin, lhe structures ot the proaucts are identified by FT-IR and ^1H NMR. The derivatives of cyclodextrin are used to catalyze the dehydrogenation of catechols to orthoquinones, and their activities are assayed by spectrophotometer. The results show that both β-CD and β-CD-His can catalyze catechol oxidation, and the activity becomes stronger with attaching copper ion to the cyclodextrin primary face.

关 键 词：环糊精 多酚氧化酶 模拟酶 催化 

分 类 号：Q554.1[生物学—生物化学]