人胃癌细胞系BGC-823中SP细胞的耐药性研究  

A Study on the Drug Resistance of SP Cells in Human Gastric Cancer Cell Line BGC-823

作　　者：吴松洁[1] 贾晓渊[1] 钱其军[1] 

机构地区：[1]浙江理工大学生命科学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2008年第5期573-577,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省自然科学基金重点项目(Z205618)

摘　　要：鉴定人胃癌细胞系BGC-823中的SP(side population)细胞,并进一步研究SP细胞对化疗药物5-氟尿嘧啶的耐受性。利用Hoechst33342对细胞染色,通过流式细胞仪检测及分选Hoechst低染的SP细胞;同样的方法检测5-氟尿嘧啶处理后的细胞中SP细胞比例的变化;MTT法检测分选后的SP与非SP细胞的抗药性差异。结果表明,人胃癌细胞系BGC-823中存在4%左右的SP细胞;细胞经过5-氟尿嘧啶处理后,SP细胞的比例显著升高;体外正常培养条件下SP细胞并不具备增殖优势,但分选后的SP细胞较非SP细胞显示出了更强的耐药存活能力。To identify the side population（SP）cells in human gastric cancer cell line BGC-823 and further study the chemotherapeutic drug resistance of SP cells to 5-fluorouracil. Cells are stained with Ho- echst 33342 and Flow Cytometry is used to detect and sort SP cells. After treatment with 5-fluorouracil, changes in the proportion of SP cells are detected using the same method. The differences in drug resistance between SP and non-SP cells are tested by MTT assay. Results. BGC-823 cell line contains about 4% SP cells. The proportion of SP cells increases significantly after 5-fluorouracil treatment. The sorted SP cells don＇t have the proliferation advantage in vitro, while they demonstrate the tougher viability than non- SP ones when suffering with drug treatment.

关 键 词：SP细胞 Hoechst 33342 ABCG2 多药耐药性 肿瘤干细胞 

分 类 号：Q279[生物学—细胞生物学]