复方二氧化钛软膏中防晒剂的鉴别和含量测定  

Identification and quantitative determination of sunscreen agents in compound titanium dioxide ointment

作　　者：叶光明[1] 于晓华 陈云红[1] 古丽萍[1] 姜云云[1] 吴明男[3] 

机构地区：[1]中国人民解放军101医院药剂科,无锡214044 [2]南京军区军事训练医学研究所,无锡214044 [3]蚌埠医学院药学系,蚌埠233000

出　　处：《药学服务与研究》2008年第4期261-263,共3页Pharmaceutical Care and Research

基　　金：南京军区医学科研"十一五"计划重点课题(No.06Z07)

摘　　要：目的：建立复方二氧化钛软膏中防晒剂的鉴别和含量测定方法。方法：以显色反应鉴定二氧化钛，采用HPLC法同时测定二苯酮-3（MC40）和甲氧基肉桂酸辛酯（MC80）的含量。色谱柱为Diamonsil C18柱（200min×4．6mm，5μm）；流动相为甲醇：水（90：10）；流速1．0mL／min；柱温35℃；检测波长0～9min为287nm，9～20min为309nm。结果：MC40和MC80分别在30-300μg／mL和100～1000 μg／mL范围内呈良好的线性关系。精密度、回收率、重复性和稳定性均符合方法学要求。结论：该方法准确、可靠、快速，适用于复方二氧化钛软膏的质量控制。Objective：To establish a method for identification and quantitative determination of sunscreen agents in com pound titanium dioxide ointment. Methods： Titanium dioxide was identified by color reaction. HPLC method was used for simultaneous determination of benzophenone-3 （MC40） and octyl methoxycinnamate （ MC80 ）. A Diamonsil C18 column （200 mm × 4.6 mm,5 μm） was used. The mobile phase was methanol-water（90 ： 10） at the flow rate of 1.0 mL/min. The gradient detec tion wavelengths were at 287 nm in 0 9 min and 309 nm in 9-20 min. Results：Good linearities were obtained in the ranges of 30- 300 μg/mL and 100-1 000 μg/mL for MC40 and MC80, respectively. The precision, recovery, reproducibility and stability met the requirements of methodology. Conclusion： This method is accurate, reliable, rapid and suitable for the quality control of compound titanium dioxide ointment.

关 键 词：二苯甲酮 甲氧基肉桂酸辛酯 复方二氧化钛软膏 色谱法 高压液相 防晒剂 

分 类 号：R927.2[医药卫生—药学] R986