力竭性运动对“高住低训”小鼠脑部HIF-1α蛋白表达及NOS/NO的影响  

Influence on HIF-1α Protein Expression and NOS/NO in Mouse's Brain with Exhaustive Exercise During "Living-high Training-low"

作　　者：刘建军[1] 

机构地区：[1]上海对外贸易学院体育教学部,上海201620

出　　处：《沈阳体育学院学报》2008年第4期50-52,56,共4页Journal of Shenyang Sport University

摘　　要：目的:旨在探讨"高住低训"下力竭运动对小鼠大脑内HIF-1蛋白表达和NOS/NO系统的影响。方法:30只4周雄性小鼠随机分成5组,常氧对照组(NC)、常氧训练组(NT)、低氧对照组(HC)、高住低训组(HT)、高住低训注射药物CHX组(HTC),每个训练组分别进行9周每天1h的游泳训练,最后一次力竭运动后断头处死,比较常压有氧训练和模拟"高住低训"的小鼠大脑HIF-1蛋白表达和NOS活性和NO含量的变化。结果:"高住低训"组与常压常氧训练组比较,HIF-1表达量明显减少,cNOSi、NOS活性明显提高,NO含量明显增加。结论:"高住低训"组小鼠大脑组织逐渐适应了低氧刺激。Objective：To investigate the effects of exhaustive exercise on HIF-1α protein expression, NOS activity and NO level of mice＇s brain during ＂living high-training low＂（ HiLo）. Methods： 30 four-week-old male Kunming mice were randomly assigned to 5 groups： normoxic controls （NC）, normoxic training （NT）, hypoxic controls （HC）, HiLo training （HT）, HiLo training and injected CHX （HTC）. Each training group was trained in swimming exercise for an hour per day for 9 weeks. All mice were killed after the last exhaustive exercise. Biochemical methods were employed to measure and compare the changes in HIF-1α protein expression, NOS activity and NO level in mice＇s brain between normoxic and HiLo training. Results：Compared with normoxic training group, HIF-1α protein expression declined, cNOS, iNOS activities and NO level increased most significantly in HiLo training group. Conclusion：The mice＇s brain adapted to hypoxia during HiLo training.

关 键 词：高住低训 低氧诱导因子 一氧化氮合酶 一氧化氮 

分 类 号：G804.7[文化科学—运动人体科学]