多粘菌素E高产菌株的推理选育  

Rational Selection of Colistin Over-producing Strain

作　　者：陈万河[1] 王普[1] 蒋红军[1] 宋友礼[2] 

机构地区：[1]浙江工业大学生物制药研究所 [2]上海医药工业研究院,上海200040

出　　处：《农药》2008年第9期641-643,共3页Agrochemicals

摘　　要：根据多粘菌素E生物合成途径进行高产菌株的推理选育。以多粘类芽孢杆菌(Bacillus polymgxa)C105x为出发菌株,采用紫外线诱变处理,并结合2,4-二氨基丁酸(L-DAB)和2-脱氧-D-葡萄糖(2-DOG)的抗性筛选,选育得到较佳正变株CL7-49。该菌株具有多粘菌素E发酵效价高、遗传稳定性好等特性。将该突变株应用于50m3发酵罐的生产验证,发酵效价较对照菌株C105x提高了48%。Breeding of colisfin higher-producing strain induced by UV radiation was studied. Strain Bacillus polymgxa C 105x was mutated by UV treatment for 30 s, and drug-tolerant mutants were selected by the addition of 0.2% L-DAB and 0.5% 2-DOG in screening medium. A high-producing strain CL7-49 with stable inheritance was obtained. In 50 m^3 bioreactor, the productivity of colisfin for mutant strain CL7-49 was increased by 48 % compared with original strain C105x.

关 键 词：多粘菌素 多粘类芽孢杆菌 紫外诱变 2 4-二氨基丁酸 2-脱氧-D-葡萄糖 

分 类 号：S482.2[农业科学—农药学]