大鼠脊髓半横断损伤后大脑皮质初级运动区caspase-3的表达  

Expression of Caspase-3 in Primary Motor Cortex after Hemisection Spinal Cord Injury of Rats

作　　者：温石磊[1] 齐建国[1] 章为[1] 王廷华[2] 刘希伟[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室 [2]四川大学神经疾病研究室,成都610041

出　　处：《四川解剖学杂志》2008年第3期24-26,共3页Sichuan Journal of Anatomy

摘　　要：目的探讨大鼠脊髓半横断损伤(hSCI)后大脑皮质初级运动区(M1)神经细胞的凋亡情况。方法成年健康Wistar大鼠T8-9脊髓左侧半横断术后,用RT-PCR和免疫组织化学法检测各个时间点caspase-3mRNA的表达量以及阳性细胞的分布。结果手术组中左、右两侧M1区的caspase-3mRNA表达量与假手术组比较,在6h即显著上升,1d达到高峰,3d、7d、14d组下降,而在21d又略有上升;6h组M1区表达量右侧明显高于左侧。免疫组织化学显示阳性细胞主要集中在M1的多形细胞层、锥体层。结论大鼠脊髓半横断损伤后M1细胞凋亡在早期最为剧烈,不仅导致支配损伤侧的M1区细胞凋亡,同样也导致支配未损伤侧脊髓的M1区神经细胞凋亡。Objective To explore the apoptosis status in primary motor cortex area（M1） in both sides of the brain following hSCI. Methods 56 adult healthy Wistar rats were randomly divided into 8 groups, 7 rats respectively： blank group, sham-operated control group and operation groups （6h, ld, 4d, 7d, 14d and 21d）. The rats thoracic cord were hemitransectioned in the left side at T8-9 after operation, RT-PCR and immunohistochemical methods were performed for detecting the caspase-3 expression. Results In both sides of the primary motor cortex area, the expression level of Caspase-3 mRNA in 6h group was obviously higher than that in blank group and sham-operational group. It reached the peak at ld group, then began to decline at 3d and 7d groups, and finally sightly up-regulated in 21d group. In 6h group, its expression in right side was significantly higher than that in the left. The positive expression of Caspase-3 was seen in the cytoplasm and the neural processes of primary motor cortex cells, which mainly localized in multiform cell layers and pyramidal layer. Conclusion The expression of Caspase-3 changed violently in early stage after the operation,hSCI caused M1 cells apoptosis not only in the side which dominates the injured side of the spinal cord, but also the intact side.

关 键 词：Caspase-3脊髓 初级皮质运动区RT-PCR 大鼠 

分 类 号：R651.2[医药卫生—外科学]