ATF3在单纯性尿道下裂中的表达及意义  

ATF3 is up-regulated in sporadic cases of isolated hypospadias

作　　者：李远伟[1] 赵晓昆[2] 高智勇[1] 吴万瑞[1] 樊皓明[1] 何军[3] 

机构地区：[1]湖南省人民医院泌尿外科,长沙市410005 [2]中南大学湘雅二医院泌尿外科 [3]湖南省儿童医院泌尿外科

出　　处：《临床小儿外科杂志》2008年第4期29-32,共4页Journal of Clinical Pediatric Surgery

基　　金：湖南省自然科学基金资助项目(05jj30056)和长沙市科技局基金资助项目(2006年)

摘　　要：目的研究激活转录因子3(Activating transcription factor 3,ATF3)在尿道下裂包皮组织中的表达情况,探索ATF3与散发的单纯性尿道下裂之间的关系。方法采用免疫组织化学方法,在蛋白质水平检测ATF3蛋白在散发的单纯性尿道下裂患者和对照组之间的表达情况。采用RT-PCR的方法,在RNA水平检测ATF3mRNA在两组之间的表达差异。结果ATF3蛋白在散发的单纯性尿道下裂中的阳性表达率为90%(27/30),在对照组中为10%(2/20),两组比较,差异有统计学意义(P<0.05)。散发的单纯性尿道下裂患者ATF3mRNA的相对表达量为0.5375±0.0591,对照组相对表达量为0.4193±0.0678,两组比较,差异有统计学意义。从数据上看,重型组中ATF3mRNA相对表达量高于其它两组。结论正常人包皮组织中ATF3表达量很少,散发的单纯性尿道下裂患者包皮组织中ATF3的表达明显增多,提示ATF3和散发的单纯性尿道下裂关系密切。进一步研究尿道下裂中ATF3产生的分子基础和ATF3相关的信号转导通路,有可能揭示散发的单纯性尿道下裂的病因。Objective To examine the expression of Activating transcription factor 3 （ATF3） in prepuce tissues, and to explore their relations to sporadic cases of isolated hypospadias.nethods Protein and mRNA were detected by immunohistochemistry and semiquantitative RT-PCR,we quantified the expression differences of ATF3 in hypospadic and control tissues, Results The expression of ATF3 in prepuce tissues from 30 boys with isolated hypospadias and 20 boys with normal prepuce tissue were examined by immunohistoche mistrg.Of the 50 specimens 27（90% ） of the 30 patients with hypospadias showed expression of ATF3 protein, and only 2 （10%） of the 20 patients in control group were positive （P 〈 0.05）.Detected by semiquantitative RT-PCR, The ATF3 mRNA relative expression levels was 0.5375± 0.0591,0.4193 ± 0.0678 for hypospadias samples and normal samples respectively, and the difference between them was statistically significant.The expression levels of ATF3 mRNA were stronger in patients with severe hypospadias compared with mild and moderate bypospadias. Conclusions Our results indicate that ATF3 is up-regulated in the prepuce tissues of boys with sporadic hypospadias, suggesting a role for this transcription factor in the development of this abnormality.Research in the areas of ATF3 gene may open new avenues toward increased understanding of the cause of hypospadias.

关 键 词：尿道下裂/病理学 尿激酶 基因表达 

分 类 号：R726.9[医药卫生—儿科]