浙江省温州市啮齿动物中汉坦病毒的分子流行病学研究  

The molecular epidemiologic investigation of hantavirus carried by rodent hosts in Wenzhou, Zhejiang province

作　　者：林献丹[1] 杨鹏飞[2] 廖晓伟[3] 李梅福[1] 高娜[2] 陈祎[1] 曾士典[1] 温怀加 陈玲萍[1] 李明慧[4] 张永振[4] 

机构地区：[1]浙江省温州市疾病预防控制中心,325001 [2]新疆石河子大学生命科学学院 [3]浙江省瑞安市疾病预防控制中心 [4]中国疾病预防控制中心传染病预防控制所

出　　处：《中华流行病学杂志》2008年第9期891-894,共4页Chinese Journal of Epidemiology

基　　金：科技部国家“十五”攻关资助项目（2003BA712A08-02）;本研究得到浙江天元生物药业股份有限公司的资助,谨志衷心感谢

摘　　要：目的研究浙江省温州市啮齿动物中汉坦病毒（HV）流行情况及病毒型别，为该地区肾综合征出血热（HFRS）的预防控制提供科学依据。方法采用间接免疫荧光法（IFA）检测鼠肺中HV抗原；用RT—PCR法扩增阳性样品中HV的部分S片段及部分M片段；构建系统发生树进行系统发生分析及分型。结果在温州市HFRS疫区共捕获啮齿动物96只，在6份鼠肺样品中检测到HV抗原，其中4只褐家鼠，1只黄胸鼠与1只黄毛鼠，病毒携带率为6．3％。用汉城病毒（SEOV）特异引物从其中5份HV抗原阳性样品中扩增出部分S片段（620～999nt）及部分M片段（2001～230Int）并测定序列。对扩增出的部分S及M片段的核苷酸序列分析发现，5株病毒与现有的SEOV有高同源性，均为汉城型HV。但在用部分S片段及部分M片段核苷酸序列所构建的系统进化树上，5株病毒的聚集模式不同。结论温州市的褐家鼠、黄胸鼠、黄毛鼠均携带汉城型HV，并可能发生基因片段的重排。Objective To study the epidemiological features of hantavirus in rodents in Wenzhou, Zhejiang province. Methods Rodents were captured in Wenzhou, where hemorrhagic fever with renal syndrome （HFRS） had been endemic. Hantavirus antigens in the rat lungs were detected by immunofluorescence assay （IFA）. Partial S segment （nt 620-999） and partial M segment （nt 2001-2301） sequences were amplified by RT-PCR, and then sequenced. Neighbor-joining method was used to construct for phylogenetic analysis. Results A total of 96 rodents were trapped in the epidemic areas, and 6 hantavirus antigens were identified from these lung samples （ 6.3 % ）. Partial S and partial M segment sequences were successfully recovered from 5 samples and determined. Phylogenetic analysis of these sequences indicated that all viruses belonged to Seoul virus （SEOV）, regardless of the sources （Rattus norvegicus, Rattus tanezumi and Rattus rattoide ） that they were derived. However, the clustering pattern in the partial S-tree was different from that in the partial M-tree, suggesting that the re-assortment between SEOVs had occurred. Conclusion All Rattus rats carried SEOV in Wenzhou and the genetic reassortment with SEOV had occurred naturally.

关 键 词：汉坦病毒 汉城型病毒 基因分型 系统发生分析 

分 类 号：R181[医药卫生—流行病学]