机械应力对MG63成骨样细胞结缔组织生长因子表达的影响  

Effect of cyclic mechanical stimulation on expression of connective tissue growth factor in MG63 osteoblastlike cells

作　　者：杨敏[1] 肖立伟[1] 黄凌云[1] 彭依群[1] 隋国良[1] 何玉玲[1] 崔蓉蓉[1] 黄佼[1] 卢瑛[1] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙410011

出　　处：《中华内分泌代谢杂志》2008年第4期356-359,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30572078,30600661）

摘　　要：目的观察力学刺激对MG63成骨样细胞的结缔组织生长因子（CTGF）表达的影响以及丝裂原活化蛋白激酶（MAPK）信号途径在这一过程中的作用。方法应用Western印迹法检测MG63细胞CTGF蛋白表达及细胞外信号调节激酶（ERK）、c-Jun氨基末端激酶（JNK）、p38磷酸化水平，应用RT-PCR检测CTGFmRNA表达。结果环状应力刺激可显著上调CTGF蛋白及mRNA表达，3～6h达高峰，升高2～3倍；并且激活ERK及JNK信号途径，刺激10min开始活化，ERK在60min达高峰，而JNK在15～30min达高峰，对p38信号途径没有明显活化作用。JNK信号通路阻断剂SP600125能够阻断应力刺激对CTGF的上调作用，而ERK信号阻断剂PD98059及p38信号阻断剂SB203580却无此作用。结论环状机械应力通过JNK依赖的途径上调了MG63细胞的CTGF表达。Objective To investigate cyclic mechanical stimulation on expression of connective tissue growth factor （CTGF） in osteoblast-like cells （ MG63 ） and to explore the role of MAPK involved in the process. Methods Expressions of CTGF protein and mRNA in MG63 cells were detected by Western blot and RT-PCR, respectively. Phosphorylation levels of p38, ERK, JNK were examined by Western blot. Results Cyclic mechanical stimulation upregulated expressions of CTGF protein and mRNA. The levels reached a maximal response of 2-3 fold after 3-6 h. ERK and JNK signal pathways were activated by cyclic mechanical stimulation, the phosphorylated proteins increased within 10 min of stretch, phosphorylated ERK reached maximal levels by 60 min of stretch, phosphorylated JNK reached maximal levels by 15-30 min of stretch, but not for p38 signal pathway. Only the inhibitior of JNK signal pathway （SP600125） markedly suppressed stretch-induced CTGF expression, meanwhile the inhibitors of ERK （PD98059） and p38 （SB203580） did not show such effect. Conclusion Cyclic mechanical stimulation upregulates CTGF expression via JNK-dependent pathway in MG63 cells.

关 键 词：骨质疏松 机械应力 MG63细胞 结缔组织生长因子 丝裂原活化的蛋白激酶 

分 类 号：R285.5[医药卫生—中药学]