抗氧化剂对去卵巢大鼠骨密度和血清生化指标的影响  

Effects of antioxidants on bone mineral density and biochemical markers in ovariectomized rats

作　　者：李明[1] 白晓春[2] 刘俊[3] 刘阳[4] 潘婷[1] 陈煜[2] 罗深秋[2] 

机构地区：[1]南方医科大学基础医学院实验教学管理中心,广州510515 [2]南方医科大学基础医学院细胞生物学教研室,广州510515 [3]中国人民解放军广州军区广州总医院泌尿外科 [4]南方医科大学基础医学院人体解剖与组织胚胎学教研室,广州510515

出　　处：《中华内分泌代谢杂志》2008年第4期368-371,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30300397）;广东省卫生厅基金（WSTJJ20041201170453519721104881）

摘　　要：目的研究抗氧化剂维生素C、E及还原型谷胱甘肽（GSH）不同配伍用药方法对去卵巢大鼠骨密度及血清生化指标的影响。方法4月龄雌性SD大鼠70只，随机取50只行双侧卵巢切除术，20只行假手术。3个月后随机从手术组和假手术组各取10只大鼠检测体重、子宫湿重、左侧股骨及腰椎骨密度和血清生化指标Ca^2＋、肌酐、碱性磷酸酶（ALP）水平，以确定骨质疏松模型建立成功。确定模型建立成功后，其余动物分为A（假手术）、B（去卵巢生理盐水对照组）、C（维生素C＋维生素E）、D（GSH）、E（维生素C＋维生素E＋GSH）5组，每组10只。药物用量维生素C（750mg·kg^-1·d^-1）、GSH（125mg·kg^-1·d^-1）腹腔注射，维生素E（250mg·kg^-1·d^-1）灌胃，模型组每天以生理盐水腹腔注射。3个月后，检测各组动物左侧股骨及腰椎骨密度和血清生化指标。结果抗氧化剂治疗3个月后，与模型组相比，左侧股骨及腰椎骨密度三个治疗组均明显增加，其中D、E组增加最显著；血清ALP各组均显著降低；超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）水平和血清抑制OH^-能力D、E组显著升高；丙二醛水平C、D组显著降低；各组血清Ca^2＋水平无明显改变。结论抗氧化剂维生素C、维生素E及GSH显著增加去卵巢骨质疏松模型大鼠骨密度，阻止血清抗氧化成分（SOD、GSH-Px）减少，提高血清抑制OH^-能力，降低脂质过氧化物丙二醛。Objective To investigate the effects of different combinations of vitamin （Vit） C, Vit E and GSH on the biochemical markers and bone mineral density （ BMD ） in ovariectomized rats. Methods Seventy female SD rats aged 4 months were divided into two groups, 20 rats with sham operation in control group and 50 rats with bilateral oophorectomy in model group. 3 months later, 10 rats in the model group and 10 rats in the control group were randomly selected and their body weight, uterus weight, BMD of the left femurs and lumbar spines, biomechanical characteristics of the left femurs, serum levels of Ca^2＋, creatinine （Cr）, alkaline phosphatase （ALP）, superoxide dismutase （SOD）, malondialdehyde （MDA）, GSH-peroxidase （GSH-Px） and serum resisting abilities to OH were determined. Then the rest rats were divided into five groups： A （sham）, B （OVX control）, C （VitC＋VitE）, D （GSH） andE （VitC ＋Vit E＋GSH）. Vit C, VitEand GSH were given 750 mg./kg, 250 mg./kg, and 125 mg./kg, respectively daily for 3 months. And then the biochemical markers and BMD were measured. Results 3 months after treatment with antioxidants, BMD of left femurs and lumbars spines was increased, while the level of serum ALP was decreased markedly in B, C and D group as compared with that in B group. The serum level of SOD, GSH-Px and serum resisting ability to OH - were increased in D and E groups and the level of MDA decreased in C and D groups as compared with that in B group. Conclusion Vit C, Vit E and GSH increased BMD, prevented the decrease of SOD and GSH-Px and elevated serum resisting ability to OH^- in ovariectomized rats.

关 键 词：骨质疏松 骨密度 抗氧化剂 

分 类 号：R580[医药卫生—内分泌]