组织因子途经抑制物2基因在胰腺癌中的表达及对胰腺癌患者预后的影响  

Expression of TFPI-2 gene in pancreatic carcinoma and its prognostic significance

作　　者：汤志刚[1] 孙振阳[1] 胡何节[1] 许戈良[1] 陈炯[1] 李建生[1] 

机构地区：[1]安徽医科大学附属安徽省立医院普通外科,合肥230001

出　　处：《中华肿瘤杂志》2008年第8期598-601,共4页Chinese Journal of Oncology

基　　金：安徽省自然科学基金资助项目（050430702）

摘　　要：目的研究组织因子途径抑制物2（TFPI-2）基因在胰腺癌中的表达，探讨其对胰腺癌患者预后的影响。方法应用逆转录聚合酶链反应（RT-PCR）及Western blot技术，检测41例胰腺癌组织中TFPI-2 mRNA和蛋白的表达，分析其与胰腺癌临床病理特征的关系及对胰腺癌患者预后的影响。结果TFPI-2 mRNA和蛋白在中低分化、有神经侵犯、有淋巴转移、有血管浸润、分期晚的胰腺癌组织中的表达水平均明显低于高分化、无神经侵犯、无淋巴转移、无血管浸润、分期早的胰腺癌组织（均P〈0．05）。TFPI-2高表达组患者的术后中位生存期（12．0个月）明显长于TFPI-2低表达组（5．1个月，P〈0．05）。Cox模型分析显示，TFPI-2的表达水平可作为影响胰腺癌患者预后的独立因素。结论TFPI-2在胰腺癌中的表达与其临床分期及分化程度有关，可作为判断胰腺癌患者预后的因素。Objective To detect the expression of TFPI-2 gene in pancreatic carcinoma, and to evaluate its prognostic significance in patients with pancreatic carcinoma. Methods Reverse transcription polymerase chain reaction （RT-PCR） and Western blot were used to detect the expression of TFPI-2 mRNA and protein in the pancreatic carcinoma tissue samples from 41 patients. The correlation of its expression with clinicopathological features and its prognostic significance were analyzed. Results The expression of TFPI-2 mRNA and protein in moderately or poorly differentiated pancreatic carcinoma tissues, or in cases with nerve-involvement, lymph node and blood vessel invasion was significantly lower than that in the highly differentiated one, or without nerve involvement, or neither lymphatic nor blood vessel invasion （ P 〈 0.05 ）. The median survival time of patients with high expression of TFPI-2 （ 12.0 months） was significantly longer than that with low expression of TFPI-2 （5.1 months, P 〈0.05 ）. The Cox model analysis showed that the expression of TFPI-2 was an independent marker for prognosis in patients with pancreatic carcinoma. Conclusion The expression of TFPI-2 is correlated with clinical stage and differentiation of pancreatic carcinoma, and can be used as a prognostic marker for pancreatic carcinoma.

关 键 词：胰腺肿瘤 预后 组织因子途径抑制物2 

分 类 号：R686[医药卫生—骨科学]