采用Photoshop软件对成年SD大鼠脊髓中皮质脊髓束三维重建的研究  

USING PHOTOSHOP IN THE THREE DIMENSIONAL RECONSTRUCTION OF CORTICOSPINAL TRACTS IN THE SPINAL CORD OF ADULT SPRAGUE-DAWLEY RAT

作　　者：吕广明[1] 吴辉群[1] 汤乐民[2] 韩笑[1] 季达峰[1] 顾晓松[1] 

机构地区：[1]南通大学医学院人体解剖学教研室神经生物学研究所江苏省神经再生重点实验室,南通226001 [2]南通大学医学院影像工程实验室,南通226001

出　　处：《解剖学报》2008年第4期488-492,共5页Acta Anatomica Sinica

基　　金：江苏省高校自然科学基金项目(05KJB180105);江苏省研究生创新培养工程资助项目(CS07s-035z);江苏省神经再生重点实验室开放课题(JSK-04003)

摘　　要：目的利用大鼠脊髓连续切片,结合三维重建工具软件对正常大鼠皮质脊髓束进行三维重建。方法制作正常成年SD大鼠脊髓连续横断切片,进行Luxol快蓝（LFB）染色并摄片,获得大鼠脊髓皮质脊髓束连续切片图像。在Photoshop软件环境下完成切片图像的配准、分割、灰度化。利用3D-DOCTOR软件进行阈值分割并对大鼠脊髓、脊髓灰质、双侧皮质脊髓束结构进行三维重建,在普通台式机（PC）上对重建结构进行任意角度的观察、切割和测量。结果重建出的大鼠脊髓、脊髓灰质、双侧皮质脊髓束具有立体感,大鼠皮质脊髓束于大鼠颈1节段（C1）～骶4节段（S4）走行于大鼠脊髓后索腹侧,紧贴灰质中间带背侧,呈逐渐变细的类圆柱状形态。在PC机中进行切割后可以对切割后剩余结构进行表面积和体积的数据测量。结论利用大鼠脊髓的连续LFB染色切片,结合计算机三维重建技术,能够进行大鼠皮质脊髓束的三维重建,并提供测量数据。Objective To make three dimensional（3D） reconstruction of corticospinal tracts in rat, using serial sections of rat＇ s spinal cord and tool software for three dimensional reconstruction. Methods The serial transversal sections of rat＇s spinal cord were made and mounted directly, then stained with Luxol fast blue（LFB） and their images were taken. The section images were registered, segmented and changed into gray-scale images under the Photoshop circumstance. 3D-DOCTOR software was used to make threshold segmentation and to reconstruct the spinal cord, grey matter and bilateral cortieospinal tracts of rat. The reconstructed structures were observed at random angles, dissected and measured on a common desktop. Results The reconstructed spinal cord, grey matter, bilateral corticospinal tracts of rat were solid. In C1 to S4 segments of rat＇s spinal cord, the corticospinal tracts lay in the posterior funiculus and were close to the back of intermediate zone, appearing as gradually narrowing columns. Conclusion The corticospinal tracts in rat＇s spinal cord can be reconstructed and offer survey data by using serial LFB stained spinal cord sections and computerized three dimensional reconstruction technique.

关 键 词：皮质脊髓束 连续切片 3D-DOCTOR软件 三维重建 大鼠 

分 类 号：R323.1[医药卫生—人体解剖和组织胚胎学] R193[医药卫生—基础医学]