人脱细胞羊膜与体外培养大鼠血管平滑肌细胞的生物相容性  

BIOCOMPATIBILITY OF HUMAN ACELLULAR AMNIOTIC MEMBRANE WITH CULTURED VASCULAR SMOOTH MUSCLE CELLS IN VITRO

作　　者：王振显[1] 蔡文清[1] 瞿长宝[1] 宋永周[2] 郭威[2] 

机构地区：[1]河北医科大学第二医院泌尿外科,石家庄050051 [2]河北医科大学解剖学教研室,石家庄050017

出　　处：《解剖学报》2008年第4期598-601,共4页Acta Anatomica Sinica

摘　　要：目的了解人脱细胞羊膜(HAM)的细胞相容性;观察以HAM为生长载体,复合大鼠血管平滑肌细胞(VSMCs)构筑组织工程膀胱的可能性。方法用物理和酶消化方法处理人羊膜,将大鼠VSMCs与HAM进行体外复合培养,分别进行组织学、相差显微镜和扫描电镜观察。将20只大鼠随机分为两组后行半膀胱切除术,实验组10只行半膀胱切除后用复合大鼠VSMCs的HAM进行修补,另外10只以单纯的HAM修补。分别于术后2、4、8周测定膀胱容量并取膀胱进行组织学观察。结果经物理和酶处理的HAM纯度高,孔隙多,胶原纤维未受损;VSMCs在HAM上可黏附生长,并能长入材料的孔隙内。应用HAM行大鼠膀胱重建术后,实验组与对照组膀胱最大容量(Volmax)比较无显著性差异。组织学观察显示,2周时HAM即已吸收降解,膀胱移行细胞和平滑肌细胞开始形成,炎性反应明显;实验组膀胱平滑肌比对照组厚且规则。4周和8周时实验组和对照组膀胱均与正常膀胱组织无明显区别。结论HAM的细胞相容性良好,不影响VSMCs的形态,作为膀胱移植物进行膀胱修补吸收降解迅速,可用做组织工程膀胱的支架材料进一步研究。Objective To investigate the biocompatilility of human acellular amniotic membrane （HAM） with vascular smooth muscle cells （VSMCs） and to explore the possibility to construct tissue engineering bladder with HAM as the scaffold and VSMCs as the seed cells. Methods After physical and 1% trypsogen preparation, the HAM was mixed with VSMCs taken from rats for culture in vitro. Histological obserbation was done under inverted microscope and scanning electron microscope respectively. 20 rats were divided into two groups. Hemieystectomies were performed in 20 rats, and 10 of them were repaired with HAM grafts with VSMCs on the half bladder, the other 10 were repaired with HAM grafts without VSMCs as the control group. The rats underwent postoperative assessment of bladder volume at the 2nd,4th and 8th weeks, and the grafts were observed by light microscope at the 2nd,4th and 8th weeks afler surgery. Results The physical and 1% trypsogen treated HAM was pure with hollows and undamaged collagen fibers. The VSMCs could grow, adhere to and differentiate on the surface of HAM and into the hollows. At the 2nd,4th and 8th weeks after surgery, the bladder volumes of the experimental group were not different significantly compared with those of the control group. Epithelialization and smooth muscle cells regeneration occurred with the infiltration of inflammatory cells in the 2nd week after grafting, and the HAM were absorbed. In the 4th and 8th weeks, it was difficult to delineate the junction between the host bladder and grafts by histology. Conclusion HAM can be used as the scaffold to construct tissue engineering bladder as it has good biocompatibility with VSMCs without disturbing the cell form and the graft can be absorbed quickly.

关 键 词：平滑肌细胞 脱细胞羊膜 生物相容性 组织工程 体外培养 人 大鼠 

分 类 号：R318.08[医药卫生—生物医学工程]