催产素诱导P19细胞分化为心肌细胞的作用  

OXYTOCIN INDUCES DIFFERENTIATION OF P19 CELLS INTO CARDIOMYOCYTES

作　　者：陈炜[1] 尹青[1] 张雷[1] 赵昱[1] 张金平[1] 赵秀军[1] 孔祥增[2] 

机构地区：[1]河北医科大学组织学胚胎学教研室,石家庄050017 [2]河北医科大学第一附属医院神经内科,石家庄050017

出　　处：《解剖学报》2008年第4期611-613,共3页Acta Anatomica Sinica

摘　　要：目的研究催产素（OT）在体外诱导P19细胞分化为心肌细胞中的作用。方法将P19细胞用含OT的诱导培养基悬浮培养4d,取其形成的拟胚体（EBs）用不含OT的生长培养基贴壁培养10～12d。用免疫细胞化学、透射电镜等方法对分化的细胞进行鉴定。结果EBs周边生长晕中部分细胞变形、伸长,呈放射状排列。免疫细胞化学染色显示,变形分化的细胞表达α-横纹肌肌动蛋白（α-SCA）和心肌肌钙蛋白T（cTnT）,并以OT终浓度1×10^-7mol/L的实验组阳性率最高（P〈0.01）。透射电镜观察分化细胞具有心肌细胞发育早期的超微结构特征。结论在体外OT能够诱导P19细胞分化为早期的心肌细胞。Objective To investigate the effects of oxytocin （OT） on the differentiation of P19 cells into cardiomyocytes in vitro. Methods The P19 cells were cultivated in the medium containing different concentrations （1 × 10^-7,3 × 10^-7 and 5 × 10^-7mol/L） of OT in suspension in dishes laid with a thin layer of agar for 4 days. Then the embryonic bodies （EBs） were transferred to coverslips in 24 well plates, allowed to adhere and be cultivated in the medium without OT for 10 to 12 days. The methods of immunocytochemical staining, transmission electron microscope（TEM） were used to identify cell differentiation. Results Some border cells in the peripheral outgrowth of the experiment groups became elongate and radial, and they were a-sarcomeric actin （α-SCA） and cardiac troponin T（cTnT） positively stained. The positive cells percentages the experiment group 1 （1 × 10^-7 mol/L OT） were higher than those of other groups （ P 〈 0.01）. The results of TEM indicated that the differentiated cells had the characters of infantile cardiomyocyte. Conclusion These results above suggest that OT can induce P19 cells into infantile cardiomyocytes in vitro.

关 键 词：催产素 心肌细胞 诱导 分化 细胞培养 P19细胞 

分 类 号：Q254[生物学—细胞生物学]