早期生长反应因子-1在移植肺缺血再灌注过程中的炎症损伤作用  

The inflammatory injury role of early growth response factor in lung ischemia-reperfusion injury in rat lung transplant model

作　　者：陈乾坤[1] 姜格宁[1] 丁嘉安[1] 谢博雄[1] 陈晓峰[1] 

机构地区：[1]同济大学附属上海市肺科医院胸外科,200433

出　　处：《中华器官移植杂志》2008年第8期464-467,共4页Chinese Journal of Organ Transplantation

摘　　要：目的探讨早期生长反应因子-1（EGR-1）在移植肺缺血再灌注过程中的炎症损伤作用。方法选择SD大鼠作为供、受者，建立左肺移植模型。实验分为4组：再灌注1h组、再灌注2h组和再灌注4h组，移植后对移植肺分别进行1、2和4h的再灌注；选取未进行移植和再灌注的供肺作为对照组。采用荧光定量实时聚合酶链法检测各组肺组织中EGR-1和白细胞介素（IL）-1βmRNA的相对表达量，并检测支气管肺泡灌洗液（BAL）中性粒细胞数、肺组织湿／干质量比（W／D）及髓过氧化物酶（MPO）活性等指标。结果各再灌注组EGR-1和IL-1βmRNA的相对表达量均高于对照组，并且随着再灌注时间的延长而明显增高．4组间比较，差异均有统计学意义（P〈0．05）。各组肺组织W／D、BAL中性粒细胞数以及MPO活性也均高于对照组，并且随着再灌注时间的延长而显著增高，4组间比较，差异均有统计学意义（P〈0．05），各组EGR-1mRNA与IL-1βmRNA的表达呈显著的正相关（r=0．921，P〈0．01）。各组EGR-1mRNA的表达与BAL中性粒细胞数呈显著的正相关（r=0．876，P〈0．01）。结论EGR-1由肺缺血诱导产生和表达；在肺缺血再灌注过程中，通过IL-1β的参与，EGR-1发挥重要的炎症损伤作用。Objective To investigate the inflammatory injury role of early growth response factor （EGR-1） in lung ischemia-reperfusion injury in rat lung transplant model. Methods Rat left lung transplant models were used in our study, and divided into following groups： 1 h after reperfusion, 2 h after reperfusion and 4 h after reperfusion. The nomal lung tissure served as a control group. Quantitative real-time RT-PCR was used to detect the expression of EGR-1 and IL-1β mRNA in lung tissures. Myeloperoxidase （MPO） activity, bronchoalveolar lavage （BAL） neutrophil count and the wetto-dry （W/D） lung weight ratio were recorded in every group. Results The expression of EGR-1 and IL-1β mRNA was obviously increased in groups of 1 h, 2 h and 4 h after reperfusion, and significantly higher than in nomal control group （P〈0.05）. The values of MPO activity, BAL neutrophil count and the W/D lung weight ratio were increased obviously in groups of 1 h, 2 h and 4 h after reperfusion as compared with those in the normal control group and there was significant difference between these 4 groups （P〈0. 05）. The expression of EGR-1 mRNA was significantly correlated with the IL-1β mRNA expression in every group （r= 0. 921, P〈0. 01 ）. The EGR-1 mRNA expression had a strong correlation with BAL neutrophil count （r = 0. 876, P〈0. 01）. Conclusion EGR-1 is induced by lung tissure ischemia, and may play an important role in the inflammatory injury mediated by IL-1β in lung ischemia-reperfusion injury in the rat lung transplant model.

关 键 词：肺 再灌注损伤 EGR-1 炎症损伤 

分 类 号：R655.3[医药卫生—外科学]